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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1988-4-11
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pubmed:abstractText |
The enzymatic (rat liver mitochondria) conversion of 3 beta-hydroxy-5 alpha-cholest-8(14)-en-15-one to 5 alpha-cholest-8(14)-ene-3 beta,26-diol-15-one is described. The enzymatic product was judged, on the basis of IH and 13C NMR studies, to be a 4:1 mixture of its 25R and 25S isomers. (25R)-5 alpha-Cholest-8(14)-ene-3 beta,26-diol-15-one was prepared through a five-step synthesis from (25R)-26-hydroxycholesterol. The (25R) isomer of the new compound was found to be highly active in the suppression of the levels of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity in cultured mammalian cells and to inhibit the esterification of cholesterol in jejunal microsomes.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
29
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pubmed:volume |
151
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
130-6
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:3348769-Animals,
pubmed-meshheading:3348769-Cell Line,
pubmed-meshheading:3348769-Chemical Phenomena,
pubmed-meshheading:3348769-Chemistry,
pubmed-meshheading:3348769-Cholestenes,
pubmed-meshheading:3348769-Cholestenones,
pubmed-meshheading:3348769-Cholesterol,
pubmed-meshheading:3348769-Esters,
pubmed-meshheading:3348769-Hydroxymethylglutaryl CoA Reductases,
pubmed-meshheading:3348769-Jejunum,
pubmed-meshheading:3348769-Microsomes,
pubmed-meshheading:3348769-Mitochondria, Liver,
pubmed-meshheading:3348769-Rats
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pubmed:year |
1988
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pubmed:articleTitle |
Enzymatic formation and chemical synthesis of an active metabolite of 3 beta-hydroxy-5 alpha-cholest-8(14)-en-15-one, a potent regulator of cholesterol metabolism.
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pubmed:affiliation |
Department of Biochemistry, Rice University, Houston, Texas 77251.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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