Linked Life Data

3339085

Source:http://linkedlifedata.com/resource/pubmed/id/3339085

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1988-3-4
pubmed:abstractText
We have studied the pathway of nuclear assembly from demembranated sperm chromatin by fractionating a cell-free system from Xenopus eggs (Lohka, M. J., and Y. Masui. 1983. Science (Wash. DC). 220:719-721). Both the soluble fraction and a washed vesicular fraction are required for formation of normal nuclei that initiate replication in vitro. The soluble fraction alone decondenses chromatin and the vesicular fraction alone surrounds chromatin with membranes. Both fractions are required for formation of nuclear pore complexes. Recombining these two fractions recovers approximately 100% of the nuclear assembly and DNA replication activities. Restricting the proportion of the vesicular fraction slows acquisition of the nuclear membrane and allows observation of immature nuclear pores ("prepores"). These form as arrays around and within the chromatin mass before membranes form. Subsequently membrane vesicles bind to these prepores, linking them by a single membrane throughout the chromatin mass. At the periphery this single membrane is surrounded by an outer membrane. In mature nuclei all membranes are at the periphery, the two membranes are linked by pores, and no prepores are seen. Nuclear assembly and replication are inhibited by preincubating the chromatin with the vesicular fraction. However nuclear assembly is accelerated by preincubating the condensed chromatin with the soluble fraction. This also decreases the lag before DNA replication. Initiation of DNA replication is only observed after normal nuclei have fully reassembled, increasing the evidence that replication depends on nuclear structure. The pathway of nuclear assembly and its relationship to DNA replication are discussed.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3339085-102651, http://linkedlifedata.com/resource/pubmed/commentcorrection/3339085-1033834, http://linkedlifedata.com/resource/pubmed/commentcorrection/3339085-3026635, http://linkedlifedata.com/resource/pubmed/commentcorrection/3339085-3653079, http://linkedlifedata.com/resource/pubmed/commentcorrection/3339085-3709518, http://linkedlifedata.com/resource/pubmed/commentcorrection/3339085-3779837, http://linkedlifedata.com/resource/pubmed/commentcorrection/3339085-3888407, http://linkedlifedata.com/resource/pubmed/commentcorrection/3339085-3948244, http://linkedlifedata.com/resource/pubmed/commentcorrection/3339085-3995581, http://linkedlifedata.com/resource/pubmed/commentcorrection/3339085-4554575, http://linkedlifedata.com/resource/pubmed/commentcorrection/3339085-5531374, http://linkedlifedata.com/resource/pubmed/commentcorrection/3339085-6224569, http://linkedlifedata.com/resource/pubmed/commentcorrection/3339085-6601299, http://linkedlifedata.com/resource/pubmed/commentcorrection/3339085-6609160, http://linkedlifedata.com/resource/pubmed/commentcorrection/3339085-7357605, http://linkedlifedata.com/resource/pubmed/commentcorrection/3339085-885912
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:volume
106
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-12
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Steps in the assembly of replication-competent nuclei in a cell-free system from Xenopus eggs.
pubmed:affiliation
Cancer Research Campaign Molecular Embryology Group, Department of Zoology, Cambridge, England.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't