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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1988-2-20
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pubmed:databankReference |
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pubmed:abstractText |
A cDNA containing the entire coding region for a member of carcinoembryonic antigen (CEA) gene family has been cloned from cDNA library of HLC-1 cells by immunochemical screening with the antibody specific to nonspecific crossreacting antigen (NCA). The cDNA encodes a precursor form of a polypeptide consisting of a 34-residue signal sequence, a 108-residue N-terminal (N-) domain, a 178-residue domain (NCA-I domain) and a 24-residue domain rich in hydrophobic amino acids (M-domain). Each domain has a distinct but homologous amino acid sequence to that of the corresponding domain of CEA. Unlike the coding sequences, the 3'-untranslated sequences differ markedly in the NCA and CEA cDNAs facilitating the preparation of probes that will discriminate between nucleotide sequences for CEA and NCA.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
150
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
89-96
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3337731-Amino Acid Sequence,
pubmed-meshheading:3337731-Antigens, Neoplasm,
pubmed-meshheading:3337731-Base Sequence,
pubmed-meshheading:3337731-Carcinoembryonic Antigen,
pubmed-meshheading:3337731-Cell Adhesion Molecules,
pubmed-meshheading:3337731-DNA,
pubmed-meshheading:3337731-Electrophoresis, Agar Gel,
pubmed-meshheading:3337731-Glycoproteins,
pubmed-meshheading:3337731-Humans,
pubmed-meshheading:3337731-Immunoassay,
pubmed-meshheading:3337731-Molecular Sequence Data,
pubmed-meshheading:3337731-Nucleic Acid Hybridization,
pubmed-meshheading:3337731-RNA, Neoplasm,
pubmed-meshheading:3337731-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:3337731-Sequence Homology, Nucleic Acid,
pubmed-meshheading:3337731-Tumor Cells, Cultured
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pubmed:year |
1988
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pubmed:articleTitle |
Primary structure of nonspecific crossreacting antigen (NCA), a member of carcinoembryonic antigen (CEA) gene family, deduced from cDNA sequence.
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pubmed:affiliation |
Suntory Institute for Biomedical Research, Osaka, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study
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