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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1977-11-25
|
| pubmed:abstractText |
The laboratory methods available for the isolation and identification of Nairobi sheep disease virus have been compared. The results show that inoculation of tissue culture (BHK 21 C 13) with suspensions of infected organs or plasma followed by fluorescent antibody tests on coverslip preparations gave the quickest means of identification. This test did not depend on the production of a cytopathic effect. Primary isolation of the virus in infant mouse brain and identification either by fluorescent antibody methods or by complement fixation with antigen prepared from the mouse brain offers a slightly more sensitive isolation system and would be recommended where no tissue culture facility exists.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0049-4747
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
9
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
75-80
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:333678-Animals,
pubmed-meshheading:333678-Arboviruses,
pubmed-meshheading:333678-Cells, Cultured,
pubmed-meshheading:333678-Complement Fixation Tests,
pubmed-meshheading:333678-Cytopathogenic Effect, Viral,
pubmed-meshheading:333678-Fluorescent Antibody Technique,
pubmed-meshheading:333678-Mice,
pubmed-meshheading:333678-Nairobi Sheep Disease,
pubmed-meshheading:333678-Sheep
|
| pubmed:year |
1977
|
| pubmed:articleTitle |
The laboratory diagnosis of Nairobi sheep disease.
|
| pubmed:publicationType |
Journal Article
|