Linked Life Data

3334846

Source:http://linkedlifedata.com/resource/pubmed/id/3334846

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1988-2-11
pubmed:abstractText
Unsaturated fatty acyl chains of Dunaliella salina membrane lipids can be catalytically reduced by the homogeneous hydrogenation catalyst palladium di(sodium alizarine monosulphonate), Pd(QS)2, under conditions permitting full recovery of the cells within 24 h. The hydrogenation is accomplished by incubation of cells with the hydride form of Pd(QS)2 under 1 atmosphere of H2 and for 2 min or less. Following this protocol, hydrogenation reduces only those fatty acids located in the plasma membrane and other membranes located near the cell surface. The limited reactivity in vivo is due to the fact the Pd(QS)2 permeates into the living cells more slowly than it does into liposomes prepared from extracted Dunaliella membrane lipids. While Dunaliella is completely unaffected by exposure to the oxygenated, inactive catalyst, hydrogenated cells cease growth for approximately 12 h, during which time the hydrogenated acyl chains are being enzymatically retroconverted to their original unsaturated form. When the lipid composition approaches its prehydrogenation values, growth resumes, presumably due to the restoration of normal membrane functions. The system shows promise for studying the metabolic regulation of membrane microviscosity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
13
pubmed:volume
937
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
42-50
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Recovery of Dunaliella salina cells following hydrogenation of lipids in specific membranes by a homogeneous palladium catalyst.
pubmed:affiliation
Department of Botany, University of Texas, Austin 78713.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't