Linked Life Data

3329709

Source:http://linkedlifedata.com/resource/pubmed/id/3329709

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1988-6-14
pubmed:abstractText
Pim-1 is a putative oncogene which was discovered as a target for transcriptionally activating proviral insertions in T-cell lymphomas in mice. Its genomic and cDNA sequence have been published (Selten et al., 1986, Cell 46, 603-611). We have isolated and sequenced pim-1 cDNA clones from the human leukemia cell line K562 (h-pim-1). Comparison of human and mouse cDNA sequences reveals that both contain: (i) a GC rich leader sequence, (ii) an open reading frame encoding a 313 amino acid protein (94% conserved) showing obvious homology to protein kinases and (iii) a 1.3 kb 3' untranslated region with two polyadenylation signals and five copies of the mRNA destabilizing motif ATTTA. "In vitro" translation experiments show that both mouse and human cDNA derived RNA can translate into a protein of the expected size. Their identity was confirmed by immuno-precipitation with antisera raised against synthetic pim-1 oligopeptides.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0890-6467
pubmed:author
pubmed:issnType
Print
pubmed:volume
1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
103-12
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Comparison of the human and mouse PIM-1 cDNAs: nucleotide sequence and immunological identification of the in vitro synthesized PIM-1 protein.
pubmed:affiliation
Division of Molecular Genetics, The Netherlands Cancer Institute, Amsterdam.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't