Linked Life Data

3327542

Source:http://linkedlifedata.com/resource/pubmed/id/3327542

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1988-5-3
pubmed:abstractText
The release of relaxin from cultured porcine luteal cells (derived from pregnant sows) was detected by a reverse hemolytic plaque assay. In this assay, luteal cells are cocultured in monolayers with protein-A-coupled ovine erythrocytes. In the presence of porcine relaxin antiserum and complement, a zone of hemolysis--a plaque--develops around relaxin-releasing luteal cells. Treatment with a prostacyclin analogue (carba-prostacyclin; 10 and 100 ng/ml) significantly accelerated the rate of plaque formation in a dose-dependent manner. In contrast, the culture of luteal cells in the presence of cyclooxygenase inhibitors (indomethacin, ibuprofen, and diclofenac sodium) resulted in no change in the rate of basal relaxin release. These results add weight to the view that prostacyclin may contribute alone, or perhaps in combination with other prostanoids, to the overall regulation of ovarian endocrine function and relaxin release during pregnancy in the pig. However, the data are inconsistent with the possibility that prostacyclin arises from relaxin-releasing luteal cells themselves.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0006-3363
pubmed:author
pubmed:issnType
Print
pubmed:volume
37
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1241-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Prostacyclin stimulates relaxin release from cultured porcine luteal cells.
pubmed:affiliation
Department of Veterinary Physiology and Pharmacology, Iowa State University, Ames 50011.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.