Linked Life Data

3322507

Source:http://linkedlifedata.com/resource/pubmed/id/3322507

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1988-3-4
pubmed:abstractText
L-Homocysteate (L-HCA) neurotoxicity was quantitatively studied in dissociated cell cultures prepared from the fetal mouse neocortex. Five minute exposure to 3 microM-1 mM L-HCA was associated with neuronal cell loss, but not glial cell loss; the extent of neuronal damage was dependent on the concentration of L-HCA, with an ED50 of approximately 40 microM. The stereoisomer D-HCA was a somewhat weaker neurotoxin than L-HCA. Ion substitution experiments suggested that L-HCA neurotoxicity can be separated into two components on the basis of differences in time course and ionic dependence: an acute, sodium-dependent 'excitotoxic' component, marked by rapid early cell swelling; and a late, calcium-dependent component, marked by delayed cell degeneration. L-HCA neurotoxicity could be attenuated by 2-amino-5-phosphonovalerate (APV), ketamine, and kynurenate, but not by L-glutamate diethyl ester or gamma-D-glutamylaminomethyl sulfonate, consistent with a predominant involvement of N-methyl-D-aspartate receptors. APV and ketamine produced different effects on the L-HCA concentration-toxicity relation, the former drug consistent with a competitive, and the latter drug consistent with a non-competitive, mechanism of antagonism.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:day
22
pubmed:volume
437
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
103-10
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
L-homocysteate is a potent neurotoxin on cultured cortical neurons.
pubmed:affiliation
Department of Neurology, Stanford University Medical Center, CA 94305.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't