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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
24
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pubmed:dateCreated |
1988-2-23
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pubmed:databankReference |
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pubmed:abstractText |
The gene for Escherichia coli leucyl-tRNA synthetase leuS has been cloned by complementation of a leuS temperature sensitive mutant KL231 with an E.coli gene bank DNA. The resulting clones overexpress leucyl-tRNA synthetase (LeuRS) by a factor greater than 50. The DNA sequence of the complete coding regions was determined. The derived N-terminal protein sequence of LeuRS was confirmed by independent protein sequencing of the first 8 aminoacids. Sequence comparison of the LeuRS sequence with all aminoacyl-tRNA synthetase sequences available reveal a significant homology with the valyl-, isoleucyl- and methionyl-enzyme indicating that the genes of these enzymes could have derived from a common ancestor. Sequence comparison with the gene product of the yeast nuclear NAM2-1 suppressor allele curing mitochondrial RNA maturation deficiency reveals about 30% homology.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3320963-1103137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3320963-1108000,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3320963-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3320963-2942398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3320963-2997739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3320963-3010776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3320963-3029694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3320963-3034607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3320963-3096385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3320963-375009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3320963-382994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3320963-386276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3320963-388356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3320963-448736,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3320963-4562753,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3320963-4612023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3320963-4942762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3320963-4986473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3320963-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3320963-6094501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3320963-6248238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3320963-6323254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3320963-6341462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3320963-6345791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3320963-6390679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3320963-6751939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3320963-6956859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3320963-724245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3320963-7265238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3320963-803646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3320963-94251
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
23
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10199-210
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3320963-Amino Acid Sequence,
pubmed-meshheading:3320963-Amino Acids,
pubmed-meshheading:3320963-Amino Acyl-tRNA Synthetases,
pubmed-meshheading:3320963-Bacterial Proteins,
pubmed-meshheading:3320963-Base Sequence,
pubmed-meshheading:3320963-Cloning, Molecular,
pubmed-meshheading:3320963-Codon,
pubmed-meshheading:3320963-DNA, Bacterial,
pubmed-meshheading:3320963-Escherichia coli,
pubmed-meshheading:3320963-Gene Expression Regulation,
pubmed-meshheading:3320963-Genes, Bacterial,
pubmed-meshheading:3320963-Leucine-tRNA Ligase,
pubmed-meshheading:3320963-Molecular Sequence Data,
pubmed-meshheading:3320963-Saccharomyces cerevisiae
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pubmed:year |
1987
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pubmed:articleTitle |
Molecular cloning and nucleotide sequence of the gene for Escherichia coli leucyl-tRNA synthetase.
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pubmed:affiliation |
European Molecular Biology Laboratory, Grenoble, France.
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pubmed:publicationType |
Journal Article,
Comparative Study
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