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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1988-2-9
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pubmed:abstractText |
Human c-myc protein, p62c-myc, has been quantitated by flow cytometry in the nuclei of normal marrow and peripheral blood cells, and the HL60 cell line. Marrow and peripheral blood cells exhibit nuclear c-myc protein throughout the cell-cycle, at an average level 2-3-fold lower than HL60 cells. In no cells did p62c-myc vary more than 2-fold throughout the cell cycle. A small subset of marrow G0/G1 cells, enriched in early myeloid and blast cell fractions, contained p62c-myc at levels equal to or even exceeding those of HL60. Overall c-myc protein content was higher in myeloid, compared to erythroid and lymphoid marrow fractions. Within the myeloid lineage, the highest average p62c-myc level was present in cells of intermediate maturation, i.e. myelocytes and metamyelocytes. In the erythroid lineage, c-myc protein level was highest in the most immature cells and declined with maturation. Significant amounts of p62c-myc were present in post-mitotic, end-stage neutrophils, but were barely detectable in cycling late erythroblasts or in quiescent lymphocytes and monocytes. HL60 cells, despite c-myc gene amplification and increased gene expression, contain c-myc protein at a level corresponding to promyelocytes in normal human marrow. The virtual absence of p62c-myc in cycling late erythroblasts, and its presence in post-mitotic end-stage granulocytes, suggests that c-myc protein may have functions unrelated to cell proliferation.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0007-1048
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
67
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
293-300
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3318911-Bone Marrow Cells,
pubmed-meshheading:3318911-Cell Cycle,
pubmed-meshheading:3318911-Cell Differentiation,
pubmed-meshheading:3318911-DNA,
pubmed-meshheading:3318911-Hematopoietic Stem Cells,
pubmed-meshheading:3318911-Humans,
pubmed-meshheading:3318911-Nuclear Proteins,
pubmed-meshheading:3318911-Proto-Oncogene Proteins,
pubmed-meshheading:3318911-Proto-Oncogene Proteins c-myc
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pubmed:year |
1987
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pubmed:articleTitle |
Nuclear c-myc protein, maturation, and cell-cycle status of human haemopoietic cells.
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pubmed:affiliation |
Department of Haematology, University of Wales, College of Medicine, Cardiff.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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