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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1988-1-19
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pubmed:abstractText |
We have examined the replication and segregation of the Saccharomyces cerevisiae 2 microns circle. The amplification of the plasmid at low copy numbers requires site-specific recombination between the 2 microns inverted repeat sequences catalyzed by the plasmid-encoded FLP gene. No other 2 microns gene products are required. The overexpression of FLP in a strain carrying endogenous 2 microns leads to uncontrolled plasmid replication, longer cell cycles, and cell death. Two different assays show that the level of Flp activity decreases with increasing 2 microns copy number. This regulation requires the products of the REP1 and REP2 genes. These gene products also act together to ensure that 2 microns molecules are randomly segregated between mother and daughter cells at cell division.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3316982-341150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3316982-3524855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3316982-3525993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3316982-375221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3316982-3939256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3316982-6092912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3316982-6162838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3316982-6251374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3316982-6273866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3316982-6310326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3316982-6317192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3316982-6341357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3316982-6354471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3316982-6366510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3316982-6752695,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3316982-6757864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3316982-6883512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3316982-7035822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3316982-7049831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3316982-7116450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3316982-7407923
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3566-73
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3316982-DNA, Fungal,
pubmed-meshheading:3316982-DNA Replication,
pubmed-meshheading:3316982-Fungal Proteins,
pubmed-meshheading:3316982-Gene Expression Regulation,
pubmed-meshheading:3316982-Genes,
pubmed-meshheading:3316982-Genes, Fungal,
pubmed-meshheading:3316982-Mitosis,
pubmed-meshheading:3316982-Plasmids,
pubmed-meshheading:3316982-Recombinant Fusion Proteins,
pubmed-meshheading:3316982-Recombination, Genetic,
pubmed-meshheading:3316982-Saccharomyces cerevisiae
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pubmed:year |
1987
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pubmed:articleTitle |
Roles of the 2 microns gene products in stable maintenance of the 2 microns plasmid of Saccharomyces cerevisiae.
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pubmed:affiliation |
Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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