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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1987-12-14
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pubmed:abstractText |
The poly(A)-binding protein (PAB) gene of Saccharomyces cerevisiae is essential for cell growth. A 66-amino acid polypeptide containing half of a repeated N-terminal domain can replace the entire protein in vivo. Neither an octapeptide sequence conserved among eucaryotic RNA-binding proteins nor the C-terminal domain of PAB is required for function in vivo. A single N-terminal domain is nearly identical to the entire protein in the number of high-affinity sites for poly(A) binding in vitro (one site with an association constant of approximately 2 X 10(7) M-1) and in the size of the binding site (12 A residues). Multiple N-terminal domains afford a mechanism of PAB transfer between poly(A) strands.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3313012-13433590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3313012-3329728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3313012-3486003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3313012-3518950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3313012-3537727,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3313012-353875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3313012-3837854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3313012-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3313012-6273866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3313012-6310317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3313012-6388631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3313012-6394957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3313012-6758843,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3313012-6833379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3313012-6929525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3313012-7317363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3313012-993212
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3268-76
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1987
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pubmed:articleTitle |
A single domain of yeast poly(A)-binding protein is necessary and sufficient for RNA binding and cell viability.
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pubmed:affiliation |
Department of Cell Biology, Stanford University School of Medicine, California 94305.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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