| pubmed-article:3312466 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3312466 | lifeskim:mentions | umls-concept:C0006675 | lld:lifeskim |
| pubmed-article:3312466 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:3312466 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:3312466 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:3312466 | lifeskim:mentions | umls-concept:C0033085 | lld:lifeskim |
| pubmed-article:3312466 | lifeskim:mentions | umls-concept:C0030281 | lld:lifeskim |
| pubmed-article:3312466 | lifeskim:mentions | umls-concept:C0021641 | lld:lifeskim |
| pubmed-article:3312466 | lifeskim:mentions | umls-concept:C0030685 | lld:lifeskim |
| pubmed-article:3312466 | lifeskim:mentions | umls-concept:C0237881 | lld:lifeskim |
| pubmed-article:3312466 | lifeskim:mentions | umls-concept:C0680255 | lld:lifeskim |
| pubmed-article:3312466 | lifeskim:mentions | umls-concept:C0391871 | lld:lifeskim |
| pubmed-article:3312466 | lifeskim:mentions | umls-concept:C1283071 | lld:lifeskim |
| pubmed-article:3312466 | lifeskim:mentions | umls-concept:C1963578 | lld:lifeskim |
| pubmed-article:3312466 | lifeskim:mentions | umls-concept:C0750502 | lld:lifeskim |
| pubmed-article:3312466 | lifeskim:mentions | umls-concept:C1550605 | lld:lifeskim |
| pubmed-article:3312466 | lifeskim:mentions | umls-concept:C0456205 | lld:lifeskim |
| pubmed-article:3312466 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:3312466 | pubmed:dateCreated | 1987-12-16 | lld:pubmed |
| pubmed-article:3312466 | pubmed:abstractText | Pancreatic islets containing more than 90% beta cells from obese-hyperglycaemic (ob/ob) mice were cultured for 3 days in different concentrations of Ca2+ and glucose to evaluate the importance of intracellular Ca2+ sequestration in glucose-induced insulin release. The islet contents of calcium (total and exchangeable) and immunoreactive insulin were compared with the insulin secretory response to glucose after culture. The turnover of Ca2+ increased with increasing concentrations of glucose and Ca2+. Islets cultured in the presence of 5.5 mmol glucose/l contained more calcium and insulin than those cultured with 1 or 20 mmol glucose/l. During culture in 20 mmol glucose/l, a lowering of the Ca2+ concentration of the medium from 0.42 to 0.025 mmol/l resulted in a paradoxical increase in intracellular calcium, with improvement of the subsequent secretory response to the sugar. When the islets had been exposed to the calcium channel blocker D-600 during culture in a Ca2+-deficient medium, substantial insulin release was noted from islets containing relatively small amounts of calcium. The results suggest that the well-established role of glucose in maintaining insulin release is associated with an ability of the sugar to stimulate the retention of calcium in beta cells. | lld:pubmed |
| pubmed-article:3312466 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3312466 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3312466 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3312466 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3312466 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3312466 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3312466 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3312466 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3312466 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3312466 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:3312466 | pubmed:issn | 0022-0795 | lld:pubmed |
| pubmed-article:3312466 | pubmed:author | pubmed-author:BergstenPP | lld:pubmed |
| pubmed-article:3312466 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3312466 | pubmed:volume | 115 | lld:pubmed |
| pubmed-article:3312466 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3312466 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3312466 | pubmed:pagination | 27-34 | lld:pubmed |
| pubmed-article:3312466 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
| pubmed-article:3312466 | pubmed:meshHeading | pubmed-meshheading:3312466-... | lld:pubmed |
| pubmed-article:3312466 | pubmed:meshHeading | pubmed-meshheading:3312466-... | lld:pubmed |
| pubmed-article:3312466 | pubmed:meshHeading | pubmed-meshheading:3312466-... | lld:pubmed |
| pubmed-article:3312466 | pubmed:meshHeading | pubmed-meshheading:3312466-... | lld:pubmed |
| pubmed-article:3312466 | pubmed:meshHeading | pubmed-meshheading:3312466-... | lld:pubmed |
| pubmed-article:3312466 | pubmed:meshHeading | pubmed-meshheading:3312466-... | lld:pubmed |
| pubmed-article:3312466 | pubmed:meshHeading | pubmed-meshheading:3312466-... | lld:pubmed |
| pubmed-article:3312466 | pubmed:meshHeading | pubmed-meshheading:3312466-... | lld:pubmed |
| pubmed-article:3312466 | pubmed:meshHeading | pubmed-meshheading:3312466-... | lld:pubmed |
| pubmed-article:3312466 | pubmed:meshHeading | pubmed-meshheading:3312466-... | lld:pubmed |
| pubmed-article:3312466 | pubmed:year | 1987 | lld:pubmed |
| pubmed-article:3312466 | pubmed:articleTitle | Significance of the calcium content of mouse beta cells in the preservation of glucose-induced insulin release during culture. | lld:pubmed |
| pubmed-article:3312466 | pubmed:affiliation | Department of Medical Cell Biology, University of Uppsala, Biomedicum, Sweden. | lld:pubmed |
| pubmed-article:3312466 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3312466 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:3312466 | lld:pubmed |
