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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2-3
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pubmed:dateCreated |
1987-12-17
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pubmed:abstractText |
A gene encoding the mature form of human growth hormone (hGH) was fused to the secretion signal coding sequence of the Escherichia coli heat-stable enterotoxin II (STII). This hybrid gene was preceded by two Shine-Dalgarno sequences derived from the trp and STII-coding genes and was expressed in E. coli under the transcriptional control of the E. coli alkaline phosphatase (phoA) promoter. In low-phosphate growth media, cells synthesized about 15 to 25 micrograms of hGH/ml/1 A550 unit of cells. This represents 6 to 10% of total cellular protein. The majority of the hGH produced (more than 90%) was processed precisely and secreted into the periplasmic space. These results demonstrate that E. coli cells are able to synthesize and secrete high levels of this human protein using a prokaryotic signal sequence.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0378-1119
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
55
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
189-96
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:3311882-Chorionic Gonadotropin,
pubmed-meshheading:3311882-Escherichia coli,
pubmed-meshheading:3311882-Genes,
pubmed-meshheading:3311882-Genes, Synthetic,
pubmed-meshheading:3311882-Genetic Vectors,
pubmed-meshheading:3311882-Humans,
pubmed-meshheading:3311882-Promoter Regions, Genetic,
pubmed-meshheading:3311882-Protein Processing, Post-Translational,
pubmed-meshheading:3311882-Protein Sorting Signals,
pubmed-meshheading:3311882-Recombinant Fusion Proteins,
pubmed-meshheading:3311882-Recombinant Proteins,
pubmed-meshheading:3311882-Regulatory Sequences, Nucleic Acid
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pubmed:year |
1987
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pubmed:articleTitle |
High-level secretion of human growth hormone by Escherichia coli.
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pubmed:affiliation |
Department of Cell Genetics, Genentech, Inc., South San Francisco, CA 94080.
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pubmed:publicationType |
Journal Article
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