Linked Life Data

3311749

Source:http://linkedlifedata.com/resource/pubmed/id/3311749

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1987-12-8
pubmed:abstractText
Genes different from those of the narGHI operon and encoding a nitrate reductase activity have been cloned by Bonnefoy et al. (unpublished results). We have shown by the use of well-known assay methods that the encoded enzyme activity is catalyzed by a true nitrate reductase and not by trimethylamine-N-oxide reductase or formate dehydrogenase. The biochemical and immunological study, employing anti-(nitrate reductase) serum raised against the known enzyme, revealed that Escherichia coli contains a second nitrate reductase (nitrate reductase Z) which shares some similarities as well as differences with the known enzyme. By using a strain with a deletion of the narGHI operon and carrying a multicopy plasmid having the nitrate reductase Z genes, we have shown that nitrate reductase Z is a membrane-bound molybdoenzyme able to couple formate oxidation with nitrate reduction. Like the known nitrate reductase, this enzyme has chlorate reductase activity. The molecular mass and pH and temperature dependence of enzyme Z are similar to these of the known enzyme. On the other hand the two enzymes have significant difference in their electrophoretic mobility on polyacrylamide gels. Unlike the known enzyme, enzyme Z is synthesized in small amounts; the expression of its structural genes does not seem to be induced by nitrate, repressed by oxygen or activated by the product of the fnr gene. The immunological comparison of the two enzymes was performed by rocket immunoelectrophoresis, double diffusion on agar plates and immunoblots. These techniques disclosed a difference between the two enzymes in their recognition by the antiserum and showed that E. coli has two types of nitrate reductase.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0014-2956
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
168
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
451-9
pubmed:dateRevised
2007-7-23
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Biochemical and immunological evidence for a second nitrate reductase in Escherichia coli K12.
pubmed:affiliation
Department de Biologie, Faculte des Sciences de Luminy, Marseille, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't