Linked Life Data

3311040

Source:http://linkedlifedata.com/resource/pubmed/id/3311040

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1987-11-19
pubmed:abstractText
Migration inhibition activity from ascitic fluids of ovarian cancer patients (OC-MIF) was used to develop monoclonal antibodies. The OC-MIF was purified about 10,000 fold by affinity chromatography on L-fucose-Sepharose 6B. Spleen cells from AB/Jena mice immunized with purified OC-MIF were hybridized with P3X63 Ag 8.653 myeloma cells. Supernatants of the hybridoma cultures were screened by solid-phase binding assay, direct neutralizing assay and solid-phase RIA. Several clones of these hybridomas secreted antibodies into the culture medium, which neutralized the biological activity of OC-MIF at dilutions as high as 10(-4) relative to the initial culture medium. After expansion and cloning one clone was selected for ascitic antibody production. This monoclonal antibody coupled to Sepharose 4B adsorbed OC-MIF. Most of the adsorbed biological activity could be eluted with 0.1 M acetic acid.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0232-766X
pubmed:author
pubmed:issnType
Print
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
397-406
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Inhibition of macrophage migration by a factor from ascites fluids of ovarian cancer patients. III. Generation of monoclonal antibody specific for human MIF.
pubmed:affiliation
Zentralinstitut für Mikrobiologie und experimentelle Therapie der Akademie der Wissenschaften der Deutschen Demokratischen Republik, Jena.
pubmed:publicationType
Journal Article