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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1987-11-6
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pubmed:abstractText |
Insulin and insulin-related growth factor 1 (IGF-1) increase by 1.5-1.6-fold the rate of [3H]leucine incorporation into protein in primary monolayer cultures of chick-embryo fibroblasts (CEF); half-maximal hormone concentrations are 10 and 0.25 nM respectively. To investigate the mechanism of this effect, a rapid method is used to prepare a lysate from CEF which is active in protein synthesis. Lysate derived from cells treated for 30-150 min with insulin synthesized protein at 1.8-3.0-fold greater rate than did controls; the increased rate persisted for 20 min in vitro. Pactamycin (0.5 microM), an inhibitor of peptide-chain initiation, inhibited protein synthesis by 50% in lysates derived from insulin-treated and control cells. Thus insulin and IGF-1 cause an increase in the protein-synthesis rate in vivo, which persists in cell-free protein-synthesizing lysates of CEF.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311026-1112824,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311026-1238402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311026-14094036,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311026-2581958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311026-258971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311026-2877871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311026-4329561,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311026-4736588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311026-4818243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311026-4855594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311026-508719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311026-5114993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311026-5442278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311026-5555565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311026-558977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311026-5797378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311026-5838176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311026-6117315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311026-6166044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311026-624716,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311026-6375664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311026-6643481,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311026-6990980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311026-6991336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311026-7016179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311026-7029543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3311026-942051
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
244
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
239-42
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:3311026-Animals,
pubmed-meshheading:3311026-Cell-Free System,
pubmed-meshheading:3311026-Cells, Cultured,
pubmed-meshheading:3311026-Chick Embryo,
pubmed-meshheading:3311026-Dose-Response Relationship, Drug,
pubmed-meshheading:3311026-Fibroblasts,
pubmed-meshheading:3311026-Insulin,
pubmed-meshheading:3311026-Insulin-Like Growth Factor I,
pubmed-meshheading:3311026-Leucine,
pubmed-meshheading:3311026-Pactamycin,
pubmed-meshheading:3311026-Protein Biosynthesis,
pubmed-meshheading:3311026-Proteins,
pubmed-meshheading:3311026-Puromycin,
pubmed-meshheading:3311026-Somatomedins
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pubmed:year |
1987
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pubmed:articleTitle |
Control by insulin and insulin-related growth factor 1 of protein synthesis in a cell-free translational system from chick-embryo fibroblasts.
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pubmed:affiliation |
Howard Hughes Medical Institute Laboratories, Harvard Medical School, Massachusetts General Hospital, Boston 02114.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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