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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
19
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pubmed:dateCreated |
1987-11-4
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pubmed:abstractText |
We have developed an approach for deriving and characterizing antigen-receptor (CD3/Ti) signal-transduction mutants. This strategy combines receptor-mediated growth inhibition and fluorescence-activated cell sorting with the Ca2+-indicator indo-1. Despite the expression of structurally normal CD3/Ti complexes, one such mutant (J.CaM1) fails to exhibit inositolphospholipid metabolism or Ca2+ mobilization in response to anti-CD3 or anti-Ti monoclonal antibodies and fails to produce lymphokines in response to these antibodies. Surprisingly, anti-Ti antibody retains its effectiveness as a stimulus for the down-regulation of CD3/Ti surface expression. These cells remain responsive to AIF-4, at least one anti-CD3 antibody, and some combinations of nonagonist anti-Ti and anti-CD3 antibodies. The mutation in J.CaM1 appears to lie in a proximal component of the signal-transduction apparatus.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3309950-2428866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3309950-2439105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3309950-2578401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3309950-2578888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3309950-2939858,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3309950-2997209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3309950-3103658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3309950-3838314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3309950-3871211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3309950-3878228,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3309950-3919143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3309950-3920341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3309950-3934668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3309950-6093124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3309950-6208306,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3309950-6234599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3309950-6327821,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3309950-6982759
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
84
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6879-83
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3309950-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:3309950-Calcium,
pubmed-meshheading:3309950-Cell Line,
pubmed-meshheading:3309950-Flow Cytometry,
pubmed-meshheading:3309950-Fluorescent Antibody Technique,
pubmed-meshheading:3309950-Humans,
pubmed-meshheading:3309950-Inositol Phosphates,
pubmed-meshheading:3309950-Mutation,
pubmed-meshheading:3309950-Receptors, Antigen, T-Cell,
pubmed-meshheading:3309950-T-Lymphocytes
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pubmed:year |
1987
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pubmed:articleTitle |
Isolation and characterization of a T-lymphocyte somatic mutant with altered signal transduction by the antigen receptor.
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pubmed:affiliation |
Howard Hughes Medical Institute, Department of Medicine, University of California, San Francisco 94143.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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