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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:dateCreated |
1987-10-28
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pubmed:abstractText |
A rapid dual-column chromatographic assay for determining recombinant leukocyte interferon alpha-2 in complex mixtures is described. The assay relies on the use of a high-performance monoclonal antibody affinity column connected in tandem with a reversed-phase column. The high specificity and selectivity of these columns permits the quantitation of subcomponent species, such as interferon oligomers that may be present in assay samples. The assay has a limit of sensitivity equal to 1 microgram/g over a range of 1-20 micrograms/g. The precision of the assay was estimated to be about 5%.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9673
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
26
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pubmed:volume |
397
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
355-64
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:3308936-Chromatography, Affinity,
pubmed-meshheading:3308936-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:3308936-Escherichia coli,
pubmed-meshheading:3308936-Humans,
pubmed-meshheading:3308936-Immunosorbent Techniques,
pubmed-meshheading:3308936-Interferon Type I,
pubmed-meshheading:3308936-Leukocytes,
pubmed-meshheading:3308936-Recombinant Proteins,
pubmed-meshheading:3308936-Saccharomyces cerevisiae,
pubmed-meshheading:3308936-Sodium Dodecyl Sulfate,
pubmed-meshheading:3308936-Solutions
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pubmed:year |
1987
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pubmed:articleTitle |
Rapid dual-column chromatographic assay for recombinant leukocyte interferon alpha-2.
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pubmed:affiliation |
Process Development and Improvement Department, Interferon Sciences, Inc., New Brunswick, NJ 08901.
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pubmed:publicationType |
Journal Article
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