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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
13
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pubmed:dateCreated |
1987-11-16
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pubmed:abstractText |
Prostatic growth factor (PrGF) was purified from alkaline homogenates of human benign prostatic hyperplastic tissue by a combination of ammonium sulfate precipitation, heparin affinity chromatography, and cation-exchange chromatography. The 17,600-dalton, basic (pI 10.2) PrGF is related to basic fibroblast growth factor (bFGF) since antisera raised against synthetic peptides with sequence homologies corresponding to an internal peptide and amino- and carboxyl-terminal peptides of bFGF react with the growth factor. The growth factor appears larger than bFGF, suggesting that additional amino-terminal sequences may be present as a result of alkaline extraction in the presence of protease inhibitors.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-2960
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
30
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pubmed:volume |
26
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3843-9
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:3307914-Amino Acid Sequence,
pubmed-meshheading:3307914-Ammonium Sulfate,
pubmed-meshheading:3307914-Chromatography, Affinity,
pubmed-meshheading:3307914-Chromatography, Ion Exchange,
pubmed-meshheading:3307914-Collodion,
pubmed-meshheading:3307914-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:3307914-Fibroblast Growth Factors,
pubmed-meshheading:3307914-Humans,
pubmed-meshheading:3307914-Hyperplasia,
pubmed-meshheading:3307914-Immunoenzyme Techniques,
pubmed-meshheading:3307914-Male,
pubmed-meshheading:3307914-Molecular Weight,
pubmed-meshheading:3307914-Prostate,
pubmed-meshheading:3307914-Tissue Extracts
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pubmed:year |
1987
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pubmed:articleTitle |
Prostatic growth factor: purification and structural relationship to basic fibroblast growth factor.
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pubmed:affiliation |
Department of Urology, Medical College of Wisconsin, Milwaukee 53226.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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