Linked Life Data

3306651

Source:http://linkedlifedata.com/resource/pubmed/id/3306651

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1987-10-22
pubmed:abstractText
A method of production of choline oxidase from Cylindrocarpon-didymum which is of sufficient purity to be used in the enzymatic assaying of choline has been described. The extraction and purification techniques used were ultrasonic disintegration, tangential ultrafiltration, and chromatography on DEAE-Sepharose Fast-Flow and on Sephacryl S 200 gel. The specific activity of the preparation obtained was 7.4 UI/mg of protein. The activity recuperation yield from the cellular extract was 56%. In the course of this purification, a protein displaying catalase activity was also obtained.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0032-7484
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
283-95
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
A simplified method of production of choline oxidase suitable for choline assay.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't