Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1987-9-30
|
| pubmed:abstractText |
When rat hepatoma cells (R-Y121B) were first incubated with labeled insulin, followed by concanavalin A, at 500 micrograms/ml at 23 degrees C, the total cell-associated radioactivity increased. At 4 degrees C, however, this increase was not observed. Scatchard analysis revealed that concanavalin A increased insulin binding affinity. The bound insulin was internalized together with concanavalin A. When the cells were incubated with concanavalin A prior to insulin addition, however, the total cell-associated radioactivity decreased at both temperatures. This was caused by the masking of insulin binding sites by the lectin.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0743-5800
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
13
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
183-97
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:3304991-Animals,
pubmed-meshheading:3304991-Binding Sites,
pubmed-meshheading:3304991-Cell Membrane,
pubmed-meshheading:3304991-Cells, Cultured,
pubmed-meshheading:3304991-Concanavalin A,
pubmed-meshheading:3304991-Insulin,
pubmed-meshheading:3304991-Kinetics,
pubmed-meshheading:3304991-Liver Neoplasms, Experimental,
pubmed-meshheading:3304991-Rats,
pubmed-meshheading:3304991-Receptors, Concanavalin A
|
| pubmed:year |
1987
|
| pubmed:articleTitle |
Concanavalin A changes not only the number of insulin binding sites but also the binding affinity in rat hepatoma cells in culture.
|
| pubmed:publicationType |
Journal Article
|