Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1987-10-16
|
| pubmed:abstractText |
KU-812-F is a subclone of KU-812, which has cytological features similar to the parent clone, and which carries the Philadelphia chromosome. We studied the effects of various chemical agents and serum-free culture (SF-C) condition on its differentiation. The KU-812-F subclone differentiated into macrophage-like cells with phorbol myristate acetate (PMA), but not with other agents. Unexpectedly, under SF-C, KU-812-F cells differentiated into mature basophil-like cells, and the histamine content of cell lysates increased in proportion to maturation. The addition of the condensed supernatant of SF-C promoted the cloning efficiency of KU-812-F cells in semisolid SF-C, whereas the cloning efficiency was reduced in SF-C alone. KU-812-F cells expressed myelomonocytic antigens. Additionally, My4 was induced with PMA and SF-C, but less often. HLA-DR was not expressed in any culture conditions, despite blastic morphology of KU-812-F cells or macrophage-like cells induced with PMA. The IgE receptor could not be demonstrated under any conditions. Correspondingly, immature and mature KU-812-F cells did not respond to anti-IgE or concanavalin A (con A), while histamine release was induced with PMA. In conclusion, KU-812-F belongs to the myeloid cell lineage and is at least a bipotential cell that can differentiate into basophils and macrophage-like cells. Although the functions appear to be dissimilar to those of normal basophils, the KU-812-F cell line may be a good model for basophil differentiation. Moreover, KU-812-F cells may provide new insights because they grow in semisolid culture by an autostimulating mechanism.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0006-4971
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
70
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
612-9
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:3304457-Basophils,
pubmed-meshheading:3304457-Blast Crisis,
pubmed-meshheading:3304457-Cell Differentiation,
pubmed-meshheading:3304457-Cell Line,
pubmed-meshheading:3304457-Clone Cells,
pubmed-meshheading:3304457-Cytological Techniques,
pubmed-meshheading:3304457-Histamine,
pubmed-meshheading:3304457-Histocytochemistry,
pubmed-meshheading:3304457-Humans,
pubmed-meshheading:3304457-Hybrid Cells,
pubmed-meshheading:3304457-Karyotyping,
pubmed-meshheading:3304457-Leukemia,
pubmed-meshheading:3304457-Macrophages,
pubmed-meshheading:3304457-Microscopy, Electron,
pubmed-meshheading:3304457-Phenotype,
pubmed-meshheading:3304457-Philadelphia Chromosome
|
| pubmed:year |
1987
|
| pubmed:articleTitle |
Bipotential cell differentiation of KU-812: evidence of a hybrid cell line that differentiates into basophils and macrophage-like cells.
|
| pubmed:publicationType |
Journal Article
|