Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
1987-9-18
pubmed:databankReference
pubmed:abstractText
Glycoprotein Ib is a surface membrane glycoprotein of platelets that functions as a receptor for von Willebrand factor. It is a heterodimer composed of an alpha and a beta chain linked by a disulfide bond(s). A phage lambda gt11 cDNA expression library prepared from mRNA from a human erythroleukemia cell line, HEL, was screened using an affinity-purified antibody to the glycocalicin portion of the alpha chain of glycoprotein Ib. Eleven positive clones were isolated and plaque-purified. The largest cDNA insert was 2420 nucleotides in length and coded for a leader sequence of 16 amino acids, a mature protein of 610 amino acids, and a stop codon. It also contained 42 nucleotides of 5' noncoding sequence and 497 nucleotides of 3' noncoding sequence, including a poly(A) tail. The amino acid sequence of the alpha chain of GPIb predicted from the cDNA agreed completely with the sequence of 156 amino acids that was determined by Edman degradation of peptides isolated from human platelet glycocalicin after digestion with trypsin or Staphylococcus aureus V8 protease. The extracytoplasmic domain of the alpha subunit of GPIb contains several noteworthy structural features, including a region of seven tandem repeats of 24 amino acids that are homologous with those present in leucine-rich alpha 2-glycoprotein. The extracytoplasmic domain also contains two hydrophilic regions, one rich in charged amino acids and a second rich in serine and threonine residues. The region rich in serine and threonine includes five repeats of nine amino acids as well as the majority of the O-linked carbohydrate sites present in the molecule. The extracytoplasmic domain is followed by a potential transmembrane segment of approximately 29 amino acids and a potential intracellular domain of approximately 100 amino acids located at the carboxyl end of the molecule.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-2933256, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-2942171, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-2943738, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-3021205, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-3028640, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-3155520, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-3161731, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-3465723, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-3486420, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-3497398, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-3856868, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-4044584, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-418063, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-4504350, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-518835, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-6177045, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-6201359, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-6215034, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-6219389, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-6265903, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-6284798, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-6309792, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-6327299, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-6406478, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-641158, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-6852022, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-6956859, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-7108955, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-7316186, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-823154, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-823155, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-845165, http://linkedlifedata.com/resource/pubmed/commentcorrection/3303030-885868
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
84
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5615-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Cloning of the alpha chain of human platelet glycoprotein Ib: a transmembrane protein with homology to leucine-rich alpha 2-glycoprotein.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't