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Predicate | Object |
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rdf:type | |
lifeskim:mentions |
umls-concept:C0022262,
umls-concept:C0025663,
umls-concept:C0037813,
umls-concept:C0079240,
umls-concept:C0229671,
umls-concept:C0337438,
umls-concept:C0443196,
umls-concept:C1441414,
umls-concept:C1514811,
umls-concept:C1547902,
umls-concept:C1553874,
umls-concept:C1561577,
umls-concept:C1578820,
umls-concept:C1706245,
umls-concept:C1706462,
umls-concept:C1707455,
umls-concept:C2587213
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pubmed:issue |
8
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pubmed:dateCreated |
1987-9-10
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pubmed:abstractText |
This improved isotope-dilution gas chromatographic/mass spectrometric (GC/MS) method, in which [13C]glucose is the internal standard, meets the requirements of a Definitive Method. In a first study with five reconstituted lyophilized sera, a nested analysis of variance of GC/MS values indicated considerable among-vial variation. The CV for 32 measurements per serum ranged from 0.5 to 0.9%. However, concentration and uncertainty values (mmol/L per gram of serum) assigned to one serum by the NBS Definitive Method (7.56 +/- 0.28) were practically identical to those obtained with the proposed method (7.57 +/- 0.20). In the second study, we used twice more [13C]glucose diluent to assay four serum pools and two lyophilized sera. The CV ranged from 0.26 to 0.5% for the serum pools and from 0.28 to 0.59% for the lyophilized sera. In comparison, results by the hexokinase/glucose-6-phosphate dehydrogenase reference method agreed within acceptable limits with those by the Definitive Method but tended to be slightly higher (up to 3%) for lyophilized serum samples or slightly lower (up to 2.5%) for serum pools.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0009-9147
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
33
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1397-402
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:3301068-Blood Chemical Analysis,
pubmed-meshheading:3301068-Blood Glucose,
pubmed-meshheading:3301068-Clinical Enzyme Tests,
pubmed-meshheading:3301068-Freeze Drying,
pubmed-meshheading:3301068-Gas Chromatography-Mass Spectrometry,
pubmed-meshheading:3301068-Humans,
pubmed-meshheading:3301068-Radioisotope Dilution Technique,
pubmed-meshheading:3301068-Statistics as Topic
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pubmed:year |
1987
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pubmed:articleTitle |
Precision of glucose measurements in control sera by isotope dilution/mass spectrometry: proposed definitive method compared with a reference method.
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pubmed:publicationType |
Journal Article,
Comparative Study
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