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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1987-8-12
|
| pubmed:abstractText |
We report the production and utility of rabbit polyclonal antisera to the bacterial protein chloramphenicol acetyltransferase (CAT). The anti-CAT antibodies specifically react with CAT protein produced in bacterial or avian cells. Results reported here demonstrate that detection of CAT by immuno-dot blot techniques is at least as sensitive as the currently used in vitro enzymatic assay. The availability of antibodies to CAT offers a number of potential advantages to investigators who now use the CAT gene to study factors influencing gene regulation.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0003-2697
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
162
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
399-404
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:3300416-Acetyltransferases,
pubmed-meshheading:3300416-Animals,
pubmed-meshheading:3300416-Antibody Specificity,
pubmed-meshheading:3300416-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:3300416-Escherichia coli,
pubmed-meshheading:3300416-Genes, Bacterial,
pubmed-meshheading:3300416-Immunoassay,
pubmed-meshheading:3300416-Plasmids,
pubmed-meshheading:3300416-Quail
|
| pubmed:year |
1987
|
| pubmed:articleTitle |
An immunological assay for chloramphenicol acetyltransferase.
|
| pubmed:publicationType |
Journal Article
|