rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1987-8-12
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pubmed:abstractText |
A stable isotope dilution gas chromatography-negative ion chemical ionization-mass spectrometry assay for simultaneous quantitative measurement of 5-hydroxyeicosatetraenoic acid (HETE), 12-HETE, 15-HETE, and 12-hydroxyheptadecatrienoic acid in one single GC/MS run was established. 18O2-Labeled analogs as internal standards, prepared according to conventional procedures, were found to be useful for this application. A sample processing and derivatization sequence providing highly purified compounds with a recovery of 42.7% was elaborated. The detection limit was in the femtomole range.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/12-Hydroxy-5,8,10,14-eicosatetraenoi...,
http://linkedlifedata.com/resource/pubmed/chemical/12-hydroxy-5,8,10-heptadecatrienoic...,
http://linkedlifedata.com/resource/pubmed/chemical/15-hydroxy-5,8,11,13-eicosatetraenoi...,
http://linkedlifedata.com/resource/pubmed/chemical/5-hydroxy-6,8,11,14-eicosatetraenoic...,
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acids, Unsaturated,
http://linkedlifedata.com/resource/pubmed/chemical/Hydroxyeicosatetraenoic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Oxygen Isotopes
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0003-2697
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
162
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
337-44
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3300415-12-Hydroxy-5,8,10,14-eicosatetraenoic Acid,
pubmed-meshheading:3300415-Chromatography, High Pressure Liquid,
pubmed-meshheading:3300415-Chromatography, Thin Layer,
pubmed-meshheading:3300415-Fatty Acids, Unsaturated,
pubmed-meshheading:3300415-Gas Chromatography-Mass Spectrometry,
pubmed-meshheading:3300415-Hydroxyeicosatetraenoic Acids,
pubmed-meshheading:3300415-Indicator Dilution Techniques,
pubmed-meshheading:3300415-Isotope Labeling,
pubmed-meshheading:3300415-Oxygen Isotopes
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pubmed:year |
1987
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pubmed:articleTitle |
Quantitative measurement of 5-, 12-, and 15-hydroxyeicosatetraenoic acid together with 12-hydroxyheptadecatrienoic acid by stable isotope dilution gas chromatography-negative ion chemical ionization-mass spectrometry.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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