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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1988-7-25
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pubmed:abstractText |
We have used a serum-free culture system for enriched human hemopoietic progenitors to analyze the developmental stages and lineage specificities of the human hemopoietic colony-stimulating factors. None of the individual factors alone efficiently supported hemopoietic colony formation. Neither interleukin 3 nor granulocyte/macrophage-colony-stimulating factor alone or in combination effectively supported proliferation of progenitor cells. However, when combined with granulocyte-colony-stimulating factor or erythropoietin, these factors yielded neutrophil colonies or erythroid bursts, respectively. Serial observations of interleukin 3-supported cultures revealed sequential emergence and subsequent degeneration of clusters of cells. These observations suggest that the primary targets of interleukin 3 and granulocyte/macrophage-colony-stimulating factor are multipotent progenitors at the early stages of development rather than cells in the terminal process of maturation.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3288992-2856931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3288992-3034976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3288992-305462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3288992-3081577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3288992-3294900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3288992-3296190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3288992-3298286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3288992-3311202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3288992-3484428,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3288992-3489530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3288992-3495394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3288992-3877534,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3288992-3930522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3288992-4822532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3288992-6190911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3288992-6234038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3288992-6437231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3288992-6965346,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3288992-7059681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3288992-7358084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3288992-844518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3288992-884703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3288992-987043
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
85
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4360-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3288992-Adult,
pubmed-meshheading:3288992-Blood,
pubmed-meshheading:3288992-Bone Marrow Cells,
pubmed-meshheading:3288992-Cell Division,
pubmed-meshheading:3288992-Cell Line,
pubmed-meshheading:3288992-Cells, Cultured,
pubmed-meshheading:3288992-Colony-Stimulating Factors,
pubmed-meshheading:3288992-Culture Media,
pubmed-meshheading:3288992-Drug Synergism,
pubmed-meshheading:3288992-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:3288992-Growth Substances,
pubmed-meshheading:3288992-Hematopoietic Stem Cells,
pubmed-meshheading:3288992-Humans,
pubmed-meshheading:3288992-Interleukin-3,
pubmed-meshheading:3288992-Recombinant Proteins
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pubmed:year |
1988
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pubmed:articleTitle |
Analysis in serum-free culture of the targets of recombinant human hemopoietic growth factors: interleukin 3 and granulocyte/macrophage-colony-stimulating factor are specific for early developmental stages.
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pubmed:affiliation |
Department of Medicine, Medical University of South Carolina, Charleston.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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