Linked Life Data

328878

Source:http://linkedlifedata.com/resource/pubmed/id/328878

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1977-9-22
pubmed:abstractText
One hundred and six sera from patients with systemic lupus erythematosus (SLE), 20 from patients without SLE but with a positive FANA, and 50 controls were tested for the presence of antibodies to n-DNA, using an immunofluorescent technique with the kinetoplast of Crithidia luciliae as a substrate. A high degree of correlation existed between the results obtained with this technique and a Millipore radioimmunoassay method using a well characterized tritiated native human DNA. The result with the immunofluorescent method could be expressed semiquantitatively as a titer, if serial serum dilutions were used. Results in patients with SLE correlated well with disease activity. We conclude that the immunofluorescent technique can provide a useful alternative to a radioimmunoassay for the detection of antibodies to n-DNA. In addition, as this substrate is known to contain pure n-DNA, problems do not arise with contamination with singlestranded DNA as sometimes occurs with other test antigens. This avoids the detection of antibodies reacting only with single-stranded areas and consequently increased the specificity of the reaction.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0315-162X
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
15-20
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1977
pubmed:articleTitle
Immunofluorescent technique for the detection of antibodies to n-DNA: comparison with radioimmunoassay.
pubmed:publicationType
Journal Article, Comparative Study