Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1988-7-25
pubmed:abstractText
We describe the production and partial characterization of 12 monoclonal antibodies raised against a preparation of endoplasmic reticulum membranes obtained from Xenopus laevis liver. Four of the antibodies cross-react with liver melanocytes; two of the antibodies recognize extracellular antigens, whilst the remaining six recognize antigens present in hepatocytes. The concentrations of these latter antigens increase markedly in livers stimulated by estrogen. Western blotting analysis revealed that the six anti-hepatocyte monoclonal antibodies recognize at least five different antigens whose molecular weights are 14K, 18K, 19K, 43K, and 125K. The possible functional involvement of the various antigens in the secretory pathway was investigated using Xenopus oocytes as a surrogate secretory system. The mRNAs coding for the monoclonal antibodies were injected into oocytes and the resulting immunoglobulin chains were translated and assembled into active anti-ER antibodies inside the lumen of the ER. The effect on secretion was then observed. Our data indicate that the binding of antibodies to most antigens of the endoplasmic reticulum membrane may result in a blockage of secretion.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0014-4827
pubmed:author
pubmed:issnType
Print
pubmed:volume
176
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
221-33
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:3288484-Animals, pubmed-meshheading:3288484-Antibodies, Monoclonal, pubmed-meshheading:3288484-Cell Line, pubmed-meshheading:3288484-Endoplasmic Reticulum, pubmed-meshheading:3288484-Estradiol, pubmed-meshheading:3288484-Female, pubmed-meshheading:3288484-Hybridomas, pubmed-meshheading:3288484-Immunohistochemistry, pubmed-meshheading:3288484-Immunologic Techniques, pubmed-meshheading:3288484-Liver, pubmed-meshheading:3288484-Male, pubmed-meshheading:3288484-Mice, pubmed-meshheading:3288484-Microinjections, pubmed-meshheading:3288484-Microscopy, Fluorescence, pubmed-meshheading:3288484-Molecular Weight, pubmed-meshheading:3288484-Muramidase, pubmed-meshheading:3288484-Oocytes, pubmed-meshheading:3288484-Ovalbumin, pubmed-meshheading:3288484-RNA, Messenger, pubmed-meshheading:3288484-Vitellogenins, pubmed-meshheading:3288484-Xenopus laevis
pubmed:year
1988
pubmed:articleTitle
Effect of anti-ER antibodies within the ER lumen of living cells.
pubmed:affiliation
Department of Biological Sciences, University of Warwick, Coventry, United Kingdom.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't