Linked Life Data

3288202

Source:http://linkedlifedata.com/resource/pubmed/id/3288202

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1988-7-1
pubmed:abstractText
We have previously shown that when H4 hepatoma cells are pretreated with insulin, plant lectins, phorbol esters, or insulin mediator, the steady state concentration of gene 33 mRNA is markedly increased. The increase in gene 33 mRNA concentration with insulin is due to an increase in the transcription rate of this gene. In the present report we demonstrate that nuclear extracts prepared from H4 hepatoma cells pretreated with insulin exhibit enhanced transcription of gene 33 RNA from a DNA template containing the cap site and 1500 bp upstream of the 33 gene. This is a stable effect of insulin on the nuclear RNA polymerase II system since it is observed in frozen and thawed nuclear extracts as well as fresh nuclear extracts.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
31
pubmed:volume
153
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
116-27
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Selective transcription of an insulin-regulated gene in nuclear extracts of rat hepatoma cells.
pubmed:affiliation
Department of Pharmacology, University of Virginia Medical School, Charlottesville 22908.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't