| pubmed-article:328783 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:328783 | lifeskim:mentions | umls-concept:C0152036 | lld:lifeskim |
| pubmed-article:328783 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:328783 | lifeskim:mentions | umls-concept:C0020852 | lld:lifeskim |
| pubmed-article:328783 | lifeskim:mentions | umls-concept:C0020861 | lld:lifeskim |
| pubmed-article:328783 | lifeskim:mentions | umls-concept:C0038734 | lld:lifeskim |
| pubmed-article:328783 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:328783 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:328783 | pubmed:dateCreated | 1977-9-17 | lld:pubmed |
| pubmed-article:328783 | pubmed:abstractText | Reduction of IgM antibodies to DNA with mercaptans such as dithioerythrol or 2-mercaptoethanol completely destroys DNA binding in the Farr assay and in the immunofluorescence technique by Crithidia luciliae. In contrast reduction of IgG antibodies to DNA results in a six-fold increase of DNA binding in the Farr assay while no effect on titres in the immunofluorescence technique can be observed. Our results lead to the following conclusions: 1) The Farr assay is selective for high avidity interactions; only a minor part of IgG antibodies to DNA is measured; 2) 7S IgM antibodies to DNA cannot be demonstrated in the Farr assay or the immunofluorescence technique; obviously only multivalent interactions, as obtained with the intact 19S IgM molecule are stable in these assays; 3) reduction of IgG leads to a greater flexibility of this molecule; this facilitates monogamous bivalent binding to DNA; 4) THE PRESENTATION OF DNA in the kinetoplast of Crithidia luciliae favours a monogamous bivalent binding of antibodies to DNA with high avidity; this accounts for occasionally observed discrepancies between anti-DNA activity in the Farr assay and in the immunofluorescence technique. | lld:pubmed |
| pubmed-article:328783 | pubmed:language | eng | lld:pubmed |
| pubmed-article:328783 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:328783 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:328783 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:328783 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:328783 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:328783 | pubmed:issn | 0022-1759 | lld:pubmed |
| pubmed-article:328783 | pubmed:author | pubmed-author:AardenL ALA | lld:pubmed |
| pubmed-article:328783 | pubmed:author | pubmed-author:LakmakerFF | lld:pubmed |
| pubmed-article:328783 | pubmed:author | pubmed-author:De GrootEE | lld:pubmed |
| pubmed-article:328783 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:328783 | pubmed:volume | 16 | lld:pubmed |
| pubmed-article:328783 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:328783 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:328783 | pubmed:pagination | 143-52 | lld:pubmed |
| pubmed-article:328783 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:328783 | pubmed:meshHeading | pubmed-meshheading:328783-D... | lld:pubmed |
| pubmed-article:328783 | pubmed:year | 1977 | lld:pubmed |
| pubmed-article:328783 | pubmed:articleTitle | Immunology of DNA. VI. The effect of mercaptans on IgG and IgM anti-dsDNA. | lld:pubmed |
| pubmed-article:328783 | pubmed:publicationType | Journal Article | lld:pubmed |
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