Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1988-7-8
pubmed:abstractText
The presence of activated transforming genes was investigated in four primary aflatoxin-induced rat liver tumors in male Fischer rats, in two cell lines generated from such tumors, in an epithelial liver-derived nontransformed cell line, and in the latter cell line after transformation by aflatoxin B1 in vitro. When DNA extracted from these sources was transfected into NIH 3T3 cells, negative results were obtained from focus assays. Cotransfection of these DNA samples with a gene for resistance to G418, followed by selection for resistance to that antibiotic, and tumorigenicity testing in nude mice demonstrated DNA-mediated transfer of the neoplastic phenotype in all cases except for DNA from the nontransformed cell line. DNA extracted from these primary nude mouse tumors used in a secondary round of transfection with NIH 3T3 cells gave positive results in focus assays, which were conserved through succeeding rounds of transfection. By use of appropriate radiolabeled probes, activated ras oncogenes were detected in all samples. N-ras activation was detected in three of the primary rat liver tumors and both hepatoma cell lines. Ki-ras activation was detected in one primary rat liver tumor, and Ha-ras activation was detected in the cell line transformed in vitro with activated aflatoxin B1. The activated Ki-ras oncogene was further characterized by use of synthetic oligonucleotide probes and was shown to contain a G----A transition at the second nucleotide in codon 12.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-187331, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-273905, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-286319, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-2867825, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-2887193, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-3014349, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-3014525, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-3016723, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-3016738, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-3018922, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-3087641, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-3099282, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-3110778, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-348302, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-3510430, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-3887133, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-3923365, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-3951985, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-4064247, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-4295478, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-4318833, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-466744, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-4721221, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-6115721, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-6206054, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-6253666, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-6265801, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-6286831, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-6302839, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-6312838, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-6318112, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-6327110, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-6488453, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-7095960, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-7388840, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-768988, http://linkedlifedata.com/resource/pubmed/commentcorrection/3287372-895848
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
85
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3673-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Activation of ras oncogene in aflatoxin-induced rat liver carcinogenesis.
pubmed:affiliation
Toxicology Unit, Medical Research Council Laboratories, Carshalton, Surrey, United Kingdom.
pubmed:publicationType
Journal Article