Linked Life Data

3286254

Source:http://linkedlifedata.com/resource/pubmed/id/3286254

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1988-7-7
pubmed:abstractText
The content of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) in the photosynthetic purple sulfur bacterium, Chromatium vinosum, grown either heterotrophically or autotrophically, was highly correlated with the level of 2.0-kb mRNA encoding genes for both large (rbcL) and small (rbcS) subunits. This result indicates the transcriptional regulation of Rubisco biosynthesis in Chromatium cells. In the analysis of transcripts for rbcL and rbcS in Escherichia coli transformed by a plasmid bearing both genes downstream of E. coli tac promoter (pCKS1), the mRNAs were found to be the same sizes as those from Chromatium. However, we were unable to detect mRNA for Rubisco in E. coli harboring a plasmid containing the genes for Rubisco and its own promoter without any E. coli promoters (pCUB1). In the in vitro transcription experiment of pCKS1 and pCUB1 by E. coli RNA polymerase, it was observed that the enzyme could not recognize the Rubisco promoter. Therefore, we have purified RNA polymerase from Chromatium cells and developed a homologous in vitro transcription system. We have detected factor(s) for transcriptional regulation from either heterotrophically or autotrophically grown cells of Chromatium using the homologous in vitro transcription system.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0014-2956
pubmed:author
pubmed:issnType
Print
pubmed:day
2
pubmed:volume
173
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
483-9
pubmed:dateRevised
2007-7-23
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Transcriptional regulation of genes for plant-type ribulose-1,5-bisphosphate carboxylase/oxygenase in the photosynthetic bacterium, Chromatium vinosum.
pubmed:affiliation
Research Institute for Biochemical Regulation, School of Agriculture, Nagoya University, Japan.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't