Linked Life Data

32851

Source:http://linkedlifedata.com/resource/pubmed/id/32851

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1979-3-13
pubmed:abstractText
Intact and zona-free mouse eggs were cultured with preincubated (capacitated) spermatozoa for 1 or 4 hr. High proportions of eggs (84%--100%), examined either 1 or 4 hr after insemination, were undergoing fertilization in the intact and zona-free eggs in sperm concentration from 25--800 X 10(3) sperm/ml. The average number of spermatozoa attached to the zona pellucida and to the vitellus was only slightly increased as the sperm concentration increased. Polyspermy was increased from 25--200 X 10(3) sperm/ml but there was no clear correlation between the incidence of polyspermy and further increase of sperm concentration in both the intact and zona-free eggs. Besides a functional zona reaction, there was a definite vitelline block to further sperm entry. It seems that due to the chance collision of sperm and egg with the subsequent formation of a block mechanism in the zona pellucida and in the vitelline membrane within a short time, polyspermy cannot be increased by further increase of sperm concentrations.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0148-5016
pubmed:author
pubmed:issnType
Print
pubmed:volume
1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
267-73
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1978
pubmed:articleTitle
Relationship between sperm concentration and polyspermy in intact and zona-free mouse eggs inseminated in vitro.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.