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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1988-5-26
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pubmed:abstractText |
Proliferating cells have been immunophenotypically characterized in lymph node and bronchoalveolar lavage (BAL) samples obtained from patients with active and inactive sarcoidosis with the cell-cycle-related antigen Ki67. Ki67 monoclonal antibody was used by combined immunohistochemical methods together with antibodies recognizing macrophage- and T-cell-subset-related antigens using avidin-biotin peroxidase (ABC) and alkaline phosphatase-anti-alkaline phosphatase (APAAP) systems. Many proliferating Ki67+ cells were found in affected mediastinal lymph nodes. These cells were mainly located around granulomas and exhibited phenotypical markers of helper/inducer T cells (CD3+, CD4+). Ki67+ macrophages could not be detected in the same lesions with this technique. A different picture was found in BAL preparations where proportions of both T lymphocytes and macrophages were Ki67+. The presence of replicating lymphocytes could be correlated to disease activity, whereas the proportions of Ki67+ macrophages did not show significant differences between active and inactive disease. Interleukin-1 (IL-1) expression was investigated in the same samples with a specific antiserum. Epithelioid macrophages in granulomas and BAL macrophages in all cases exhibited cytoplasmic staining revealing an activated status. Interestingly, giant cells in granulomas were mainly devoid of IL-1 immunoreactivity. These studies support the concept that activated cells at different sites of ongoing inflammation play a central role in the mechanisms accounting for granuloma formation.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3282443-2942079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3282443-2948432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3282443-3014952,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3282443-3099617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3282443-3104393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3282443-3297354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3282443-3517219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3282443-370353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3282443-3799813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3282443-3871200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3282443-3881544,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3282443-3896081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3282443-3923038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3282443-3964815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3282443-4416280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3282443-4817856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3282443-6088134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3282443-6178290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3282443-6198355,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3282443-6206131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3282443-6221839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3282443-6361563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3282443-6454846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3282443-6460819,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3282443-6601235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3282443-6608889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3282443-6746904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3282443-6834173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3282443-6973339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3282443-6975891
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0002-9440
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
131
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pubmed:owner |
NLM
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pubmed:authorsComplete |
N
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pubmed:pagination |
191-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3282443-Adult,
pubmed-meshheading:3282443-Antibodies, Monoclonal,
pubmed-meshheading:3282443-Antigens, Surface,
pubmed-meshheading:3282443-Bronchi,
pubmed-meshheading:3282443-Female,
pubmed-meshheading:3282443-Granuloma,
pubmed-meshheading:3282443-Humans,
pubmed-meshheading:3282443-Immunoenzyme Techniques,
pubmed-meshheading:3282443-Interleukin-1,
pubmed-meshheading:3282443-Ki-67 Antigen,
pubmed-meshheading:3282443-Lymph Nodes,
pubmed-meshheading:3282443-Macrophages,
pubmed-meshheading:3282443-Male,
pubmed-meshheading:3282443-Sarcoidosis
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pubmed:year |
1988
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pubmed:articleTitle |
Immunohistochemical analysis of sarcoid granulomas. Evaluation of Ki67+ and interleukin-1+ cells.
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pubmed:affiliation |
Istituto di Anatomia Patologica, University of Verona, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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