pubmed-article:3281973 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:3281973 | lifeskim:mentions | umls-concept:C0004663 | lld:lifeskim |
pubmed-article:3281973 | lifeskim:mentions | umls-concept:C0314923 | lld:lifeskim |
pubmed-article:3281973 | lifeskim:mentions | umls-concept:C0314924 | lld:lifeskim |
pubmed-article:3281973 | lifeskim:mentions | umls-concept:C0370003 | lld:lifeskim |
pubmed-article:3281973 | lifeskim:mentions | umls-concept:C0003250 | lld:lifeskim |
pubmed-article:3281973 | lifeskim:mentions | umls-concept:C0023810 | lld:lifeskim |
pubmed-article:3281973 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
pubmed-article:3281973 | lifeskim:mentions | umls-concept:C0079603 | lld:lifeskim |
pubmed-article:3281973 | lifeskim:mentions | umls-concept:C0205210 | lld:lifeskim |
pubmed-article:3281973 | pubmed:issue | 3 | lld:pubmed |
pubmed-article:3281973 | pubmed:dateCreated | 1988-5-24 | lld:pubmed |
pubmed-article:3281973 | pubmed:abstractText | A total of 1,897 clinical specimens (1,019 aspirates and 876 swabs) were studied by indirect immunofluorescence (IF) with a mouse monoclonal antibody (MAb) against a D-galactose oligomer of Bacteroides fragilis lipopolysaccharide. The MAb has been shown to react with 96% of clinical B. fragilis isolates and with about 50% of Bacteroides ovatus and Bacteroides thetaiotaomicron isolates but not with other aerobic or anaerobic organisms tested. The sensitivity of IF in comparison with culturing was 78.9% for all three species. Of the 32 strains originating from culture-positive, IF-negative specimens, 13 lacked the target determinant for the MAb. Sensitivity was highest with specimens taken from the perineal area (87.1%) and lowest with those taken from undefined sites (56.6%). Sensitivity was better with aspirates (86.8%) than with swabs (72.6%). The specificity of IF was 95.6% for all of the material. Positive and negative predictive values were 51.1 and 98.0%, respectively. Neither long transportation times of specimens nor antimicrobial therapy seemed to correlate with the occurrence of IF-positive, culture-negative specimens. This study shows that a single MAb can be used to establish an IF assay that can complement isolation in the detection of these three members of the B. fragilis group. | lld:pubmed |
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pubmed-article:3281973 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
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pubmed-article:3281973 | pubmed:language | eng | lld:pubmed |
pubmed-article:3281973 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:3281973 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:3281973 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:3281973 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:3281973 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:3281973 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:3281973 | pubmed:month | Mar | lld:pubmed |
pubmed-article:3281973 | pubmed:issn | 0095-1137 | lld:pubmed |
pubmed-article:3281973 | pubmed:author | pubmed-author:ViljanenM KMK | lld:pubmed |
pubmed-article:3281973 | pubmed:author | pubmed-author:LehtonenO POP | lld:pubmed |
pubmed-article:3281973 | pubmed:author | pubmed-author:LinkeRR | lld:pubmed |
pubmed-article:3281973 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:3281973 | pubmed:volume | 26 | lld:pubmed |
pubmed-article:3281973 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:3281973 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:3281973 | pubmed:pagination | 448-52 | lld:pubmed |
pubmed-article:3281973 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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pubmed-article:3281973 | pubmed:year | 1988 | lld:pubmed |
pubmed-article:3281973 | pubmed:articleTitle | Detection of Bacteroides fragilis, Bacteroides thetaiotaomicron, and Bacteroides ovatus in clinical specimens by immunofluorescence with a monoclonal antibody to B. fragilis lipopolysaccharide. | lld:pubmed |
pubmed-article:3281973 | pubmed:affiliation | Department of Medical Microbiology, University of Turku, Finland. | lld:pubmed |
pubmed-article:3281973 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:3281973 | pubmed:publicationType | Comparative Study | lld:pubmed |
pubmed-article:3281973 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |