|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
1988-5-24
|
|
pubmed:abstractText |
A total of 1,897 clinical specimens (1,019 aspirates and 876 swabs) were studied by indirect immunofluorescence (IF) with a mouse monoclonal antibody (MAb) against a D-galactose oligomer of Bacteroides fragilis lipopolysaccharide. The MAb has been shown to react with 96% of clinical B. fragilis isolates and with about 50% of Bacteroides ovatus and Bacteroides thetaiotaomicron isolates but not with other aerobic or anaerobic organisms tested. The sensitivity of IF in comparison with culturing was 78.9% for all three species. Of the 32 strains originating from culture-positive, IF-negative specimens, 13 lacked the target determinant for the MAb. Sensitivity was highest with specimens taken from the perineal area (87.1%) and lowest with those taken from undefined sites (56.6%). Sensitivity was better with aspirates (86.8%) than with swabs (72.6%). The specificity of IF was 95.6% for all of the material. Positive and negative predictive values were 51.1 and 98.0%, respectively. Neither long transportation times of specimens nor antimicrobial therapy seemed to correlate with the occurrence of IF-positive, culture-negative specimens. This study shows that a single MAb can be used to establish an IF assay that can complement isolation in the detection of these three members of the B. fragilis group.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281973-1102560,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281973-1172191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281973-1254729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281973-2438749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281973-4596718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281973-4927423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281973-6108969,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281973-6120183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281973-6341482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281973-6386867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281973-659574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281973-7020409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281973-810521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281973-84899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281973-939922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281973-94651
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0095-1137
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
26
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
448-52
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
|
|
pubmed:year |
1988
|
|
pubmed:articleTitle |
Detection of Bacteroides fragilis, Bacteroides thetaiotaomicron, and Bacteroides ovatus in clinical specimens by immunofluorescence with a monoclonal antibody to B. fragilis lipopolysaccharide.
|
|
pubmed:affiliation |
Department of Medical Microbiology, University of Turku, Finland.
|
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|
