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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1988-5-26
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pubmed:abstractText |
Monoclonal and monospecific antibodies were used to characterize a major Mycoplasma pulmonis surface antigen complex, V-1. Heterogeneity of V-1 was detected among strains and a high frequency of variation was detected within subclones of single strains. Analysis of 18 different strains showed that no two displayed identical electrophoretic immunoblot patterns for V-1. Analysis of 50 filter clones from an individual strain (not previously filter cloned) revealed at least 10 different V-1 patterns. The two most frequently occurring patterns were expressed by 36% and 24%, respectively, of the total population. Serial subcloning (four separate series) of several of these original clones showed that the average rate of V-1 variation was 2 x 10(-3) per cell per generation. Immunoblots with different anti-V-1 monoclonal antibodies demonstrated that there were both structurally and antigenically different forms of this antigen. Also, two-dimensional polyacrylamide gel analyses showed that different forms of V-1 could vary in charge. This potential for variability in a major surface antigen of mycoplasmas could have important implications as to how the organism interacts with its host.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281909-113458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281909-13130825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281909-236308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281909-2418125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281909-2579158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281909-2579187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281909-3159678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281909-3312017,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281909-3570466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281909-3759235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281909-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281909-4519141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281909-4719529,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281909-5233768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281909-5340303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281909-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281909-558962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281909-5628376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281909-562852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281909-6163373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281909-6381634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281909-6690399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281909-6981830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281909-7026496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281909-7045516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281909-784892
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
56
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1358-63
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3281909-Antibodies, Monoclonal,
pubmed-meshheading:3281909-Antigenic Variation,
pubmed-meshheading:3281909-Antigens, Bacterial,
pubmed-meshheading:3281909-Antigens, Surface,
pubmed-meshheading:3281909-Bacterial Proteins,
pubmed-meshheading:3281909-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:3281909-Immunosorbent Techniques,
pubmed-meshheading:3281909-Isoelectric Point,
pubmed-meshheading:3281909-Molecular Weight,
pubmed-meshheading:3281909-Mycoplasma,
pubmed-meshheading:3281909-Species Specificity
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pubmed:year |
1988
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pubmed:articleTitle |
Heterogeneity among strains and a high rate of variation within strains of a major surface antigen of Mycoplasma pulmonis.
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pubmed:affiliation |
Department of Microbiology, University of Alabama at Birmingham 35294.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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