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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1988-4-28
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pubmed:abstractText |
The effect of insulin on glucose transport and glucose transporters was studied in perfused rat heart. Glucose transport was measured by the efflux of labelled 3-O-methylglucose from hearts preloaded with this hexose. Insulin stimulated 3-O-methylglucose transport by: (a) doubling the maximal velocity (Vmax); (b) decreasing the Kd from 6.9 to 2.7 mM; (c) increasing the Hill coefficient toward 3-O-methylglucose from 1.9 to 3.1; (d) increasing the efficiency of the transport process (k constant). Glucose transporters in enriched plasma and microsomal membranes from heart were quantified by the [3H]cytochalasin-B-binding assay. When added to normal hearts, insulin produced the following changes in the glucose transporters: (a) it increased the translocation of transporters from an intracellular pool to the plasma membranes; (b) it increased (from 1.6 to 2.7) the Hill coefficient of the transporters translocated into the plasma membranes toward cytochalasin B, suggesting the existence of a positive co-operativity among the transporters appearing in these membranes; (c) it increased the affinity of the transporters (and hence, possibly, of glucose) for cytochalasin B. The data provide evidence that the stimulatory effect of insulin on glucose transport may be due not to the sole translocation of intracellular glucose transporters to the plasma membrane, but to changes in the functional properties thereof.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281662-10976232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281662-1151775,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281662-1249055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281662-13664926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281662-13937587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281662-16993004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281662-3102556,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281662-3318814,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281662-3511051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281662-3539932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281662-3584138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281662-3882413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281662-3882703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281662-3888079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281662-4326970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281662-447648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281662-5787247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281662-6024443,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281662-623253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281662-6265437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281662-6308227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281662-6363180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281662-6376500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281662-6386808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281662-6771756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281662-6989818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281662-6990920,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281662-6994712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281662-7037054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281662-711732,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281662-786634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3281662-942051
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
250
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
277-83
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:3281662-3-O-Methylglucose,
pubmed-meshheading:3281662-Animals,
pubmed-meshheading:3281662-Biological Transport,
pubmed-meshheading:3281662-Cell Membrane,
pubmed-meshheading:3281662-Cytochalasin B,
pubmed-meshheading:3281662-Heart,
pubmed-meshheading:3281662-Insulin,
pubmed-meshheading:3281662-Intracellular Membranes,
pubmed-meshheading:3281662-Kinetics,
pubmed-meshheading:3281662-Male,
pubmed-meshheading:3281662-Methylglucosides,
pubmed-meshheading:3281662-Methylglycosides,
pubmed-meshheading:3281662-Microsomes,
pubmed-meshheading:3281662-Myocardium,
pubmed-meshheading:3281662-Rats,
pubmed-meshheading:3281662-Rats, Zucker
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pubmed:year |
1988
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pubmed:articleTitle |
Effects of insulin on glucose transport and glucose transporters in rat heart.
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pubmed:affiliation |
Faculty and Department of Medicine, University of Geneva, Switzerland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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