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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1988-4-13
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pubmed:abstractText |
We report the identification and purification of a new inflammatory monokine synthesized by the macrophage tumor cell line RAW 264.7 in response to endotoxin. This monokine, which we term "macrophage inflammatory protein" (MIP), is a doublet with an apparent molecular mass of approximately 8,000 daltons on SDS-PAGE but forms aggregates of greater than 2 x 10(6) daltons as assessed by gel filtration. Partial NH2-terminal amino acid sequence data reveal no significant homology with any previously described protein. Although the monokine is anionic under physiological conditions, it is one of two major macrophage-secreted proteins that bind to heparin at high salt concentrations. At 100 ng/ml or greater, MIP is chemokinetic for human polymorphonuclear cells and triggers hydrogen peroxide production. Subcutaneous injection of 10 ng or greater of MIP into footpads of C3H/HeJ mice elicits an inflammatory response, characterized by neutrophil infiltration. These findings suggest that MIP is an endogenous mediator that may play a role in the host responses that occur during endotoxemia and other inflammatory events.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3279154-13872176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3279154-2445780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3279154-2820532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3279154-3009619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3279154-3010124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3279154-3015137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3279154-3033074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3279154-3298433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3279154-3478107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3279154-3495587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3279154-3512598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3279154-3543052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3279154-372951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3279154-3805724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3279154-3872925,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3279154-3902871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3279154-3926894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3279154-3989315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3279154-6231310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3279154-6369481,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3279154-6384405,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3279154-6606682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3279154-90061,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3279154-942051
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
167
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
570-81
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:3279154-Amino Acid Sequence,
pubmed-meshheading:3279154-Animals,
pubmed-meshheading:3279154-Biological Agents,
pubmed-meshheading:3279154-Carrier Proteins,
pubmed-meshheading:3279154-Chemotactic Factors,
pubmed-meshheading:3279154-Female,
pubmed-meshheading:3279154-Heparin,
pubmed-meshheading:3279154-Hydrogen Peroxide,
pubmed-meshheading:3279154-Inflammation,
pubmed-meshheading:3279154-Interleukin-8,
pubmed-meshheading:3279154-Macrophages,
pubmed-meshheading:3279154-Mice,
pubmed-meshheading:3279154-Mice, Inbred C3H,
pubmed-meshheading:3279154-Molecular Sequence Data,
pubmed-meshheading:3279154-Monokines
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pubmed:year |
1988
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pubmed:articleTitle |
Macrophages secrete a novel heparin-binding protein with inflammatory and neutrophil chemokinetic properties.
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pubmed:affiliation |
Laboratory of Medical Biochemistry, Rockefeller University, New York.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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