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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1988-4-4
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pubmed:databankReference |
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pubmed:abstractText |
The nucleotide sequence of a 1.4-kilobase DNA fragment containing the alpha-acetolactate decarboxylase gene of Enterobacter aerogenes was determined. The sequence contains an entire protein-coding region of 780 nucleotides which encodes an alpha-acetolactate decarboxylase of 260 amino acids. The DNA sequence coding for alpha-acetolactate decarboxylase was placed under the control of the alcohol dehydrogenase I promoter of the yeast Saccharomyces cerevisiae in a plasmid capable of autonomous replication in both S. cerevisiae and Escherichia coli. Brewer's yeast cells transformed by this plasmid showed alpha-acetolactate decarboxylase activity and were used in laboratory-scale fermentation experiments. These experiments revealed that the diacetyl concentration in wort fermented by the plasmid-containing yeast strain was significantly lower than that in wort fermented by the parental strain. These results indicated that the alpha-acetolactate decarboxylase activity produced by brewer's yeast cells degraded alpha-acetolactate and that this degradation caused a decrease in diacetyl production.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3278689-1069307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3278689-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3278689-287063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3278689-2985470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3278689-3095618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3278689-347451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3278689-3516005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3278689-3703684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3278689-388356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3278689-395029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3278689-5447429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3278689-6277922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3278689-6310320,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3278689-6315951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3278689-6343825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3278689-6344016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3278689-6345791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3278689-6750137
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0099-2240
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
54
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
38-42
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3278689-Base Sequence,
pubmed-meshheading:3278689-Carboxy-Lyases,
pubmed-meshheading:3278689-DNA, Bacterial,
pubmed-meshheading:3278689-Enterobacter,
pubmed-meshheading:3278689-Enterobacteriaceae,
pubmed-meshheading:3278689-Fermentation,
pubmed-meshheading:3278689-Genes, Bacterial,
pubmed-meshheading:3278689-Lactates,
pubmed-meshheading:3278689-Molecular Sequence Data,
pubmed-meshheading:3278689-Plasmids,
pubmed-meshheading:3278689-Saccharomyces cerevisiae,
pubmed-meshheading:3278689-Transformation, Genetic
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pubmed:year |
1988
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pubmed:articleTitle |
Nucleotide sequence and expression of the Enterobacter aerogenes alpha-acetolactate decarboxylase gene in brewer's yeast.
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pubmed:affiliation |
Central Laboratories of Key Technology, Kirin Brewery Co., Ltd., Gunma, Japan.
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pubmed:publicationType |
Journal Article
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