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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1988-3-2
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pubmed:abstractText |
The development of a successful oral vaccine against enterotoxigenic Escherichia coli depends upon the identification of appropriate protective antigens which can be delivered effectively to intestinal mucosa. We have determined in a modified RITARD model the relative protection against intraintestinal challenge afforded by oral immunization with live enterotoxigenic E. coli carrying different candidate antigens. Studies were done with both wild-type strains and genetically manipulated strains of enterotoxigenic E. coli (parent strain E1392/75 2A) which carried plasmids containing intact heat-labile toxin (LT) gene sequences or various mutations of the LT genes. Immunizations were done by orogastric tube inoculation on days 0, 7, and 14; challenges were done on day 33. Protection against diarrhea with a homologous challenge was found to be 84 to 100% (P less than 0.01). Protection against diarrhea with challenges in which specific antigens could be tested included the following: (i) O and H antigens (O6:H16), 87 to 100% protection with different E. coli strains with identical O and H antigens (P less than 0.01) but no protection against a heterologous challenge; (ii) LT or the B subunit of LT only, approximately 50% protection (P less than 0.02). These findings suggest that O antigens are highly protective in this model but afford only serotype-specific protection and that the B subunit (with or without the A subunit) affords less protection but confers cross-protection against heterologous strains producing LT. This model should be useful in further defining appropriate protective antigens for candidate enterotoxigenic E. coli vaccine strains.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3276624-2437220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3276624-2864313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3276624-2869210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3276624-2873397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3276624-2878888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3276624-3301680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3276624-378834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3276624-4435956,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3276624-4944321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3276624-6106031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3276624-6137646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3276624-6302001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3276624-6874074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3276624-7019078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3276624-7019725
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
56
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
387-94
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3276624-Administration, Oral,
pubmed-meshheading:3276624-Animals,
pubmed-meshheading:3276624-Antigens, Bacterial,
pubmed-meshheading:3276624-Bacterial Toxins,
pubmed-meshheading:3276624-Bacterial Vaccines,
pubmed-meshheading:3276624-Cell Wall,
pubmed-meshheading:3276624-Diarrhea,
pubmed-meshheading:3276624-Enterotoxins,
pubmed-meshheading:3276624-Escherichia coli,
pubmed-meshheading:3276624-Escherichia coli Infections,
pubmed-meshheading:3276624-Escherichia coli Proteins,
pubmed-meshheading:3276624-Female,
pubmed-meshheading:3276624-Intestine, Small,
pubmed-meshheading:3276624-Male,
pubmed-meshheading:3276624-Rabbits
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pubmed:year |
1988
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pubmed:articleTitle |
Oral immunization of rabbits with enterotoxigenic Escherichia coli protects against intraintestinal challenge.
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pubmed:affiliation |
Department of Medicine, Francis Scott Key Medical Center, Baltimore, Maryland 21224.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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