| pubmed-article:3267232 | rdf:type | pubmed:Citation | lld:pubmed |
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| pubmed-article:3267232 | lifeskim:mentions | umls-concept:C2347858 | lld:lifeskim |
| pubmed-article:3267232 | pubmed:issue | 17 | lld:pubmed |
| pubmed-article:3267232 | pubmed:dateCreated | 1988-10-26 | lld:pubmed |
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| pubmed-article:3267232 | pubmed:abstractText | The organization and function of potential regulatory elements associated with the promoters of chicken H2A and H2B genes pairs have been examined. The intergene regions of six dispersed and divergently-transcribed H2A/H2B gene pairs contain several extremely well conserved and spaced blocks of sequence homology. Adjacent coding regions are on average 342 base-pairs apart. Respective TATA boxes are separated by 180 base-pairs and within this confined region there are four CCAAT boxes and a previously identified 13 base-pair H2B-specific element (H2B-box) which has homology to the octamer motif present in a number of gene promoter/enhancer elements. Transcription of H2A and H2B genes from wild-type and mutant constructs was measured in transient assays by transfection into HeLa cells, and in permanently transformed clonal cell lines. In vitro separation of the two genes at a unique intergenic site significantly decreased transcription of each gene. This suggested that the H2A/H2B gene pairs contained overlapping promoters. Deletion or point mutagenesis of the H2B-specific element decreased the levels of H2B and the H2A transcripts indicating that this sequence is a common regulatory element of both genes in the divergent-pair configeration. | lld:pubmed |
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| pubmed-article:3267232 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:3267232 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:3267232 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3267232 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:3267232 | pubmed:issn | 0305-1048 | lld:pubmed |
| pubmed-article:3267232 | pubmed:author | pubmed-author:WellsJ RJR | lld:pubmed |
| pubmed-article:3267232 | pubmed:author | pubmed-author:DaltonSS | lld:pubmed |
| pubmed-article:3267232 | pubmed:author | pubmed-author:SturmR ARA | lld:pubmed |
| pubmed-article:3267232 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3267232 | pubmed:day | 12 | lld:pubmed |
| pubmed-article:3267232 | pubmed:volume | 16 | lld:pubmed |
| pubmed-article:3267232 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3267232 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3267232 | pubmed:pagination | 8571-86 | lld:pubmed |
| pubmed-article:3267232 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:3267232 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:3267232 | pubmed:articleTitle | Conservation of histone H2A/H2B intergene regions: a role for the H2B specific element in divergent transcription. | lld:pubmed |
| pubmed-article:3267232 | pubmed:affiliation | Department of Biochemistry, University of Adelaide, South Australia. | lld:pubmed |
| pubmed-article:3267232 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3267232 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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