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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
17
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pubmed:dateCreated |
1988-10-26
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pubmed:databankReference |
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pubmed:abstractText |
The organization and function of potential regulatory elements associated with the promoters of chicken H2A and H2B genes pairs have been examined. The intergene regions of six dispersed and divergently-transcribed H2A/H2B gene pairs contain several extremely well conserved and spaced blocks of sequence homology. Adjacent coding regions are on average 342 base-pairs apart. Respective TATA boxes are separated by 180 base-pairs and within this confined region there are four CCAAT boxes and a previously identified 13 base-pair H2B-specific element (H2B-box) which has homology to the octamer motif present in a number of gene promoter/enhancer elements. Transcription of H2A and H2B genes from wild-type and mutant constructs was measured in transient assays by transfection into HeLa cells, and in permanently transformed clonal cell lines. In vitro separation of the two genes at a unique intergenic site significantly decreased transcription of each gene. This suggested that the H2A/H2B gene pairs contained overlapping promoters. Deletion or point mutagenesis of the H2B-specific element decreased the levels of H2B and the H2A transcripts indicating that this sequence is a common regulatory element of both genes in the divergent-pair configeration.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3267232-2409453,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3267232-2410771,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3267232-2411540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3267232-2411541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3267232-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3267232-2828167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3267232-2896124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3267232-3018501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3267232-3025588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3267232-3029871,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/3267232-7350544
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
12
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8571-86
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3267232-Animals,
pubmed-meshheading:3267232-Base Sequence,
pubmed-meshheading:3267232-Cell Nucleus,
pubmed-meshheading:3267232-Chick Embryo,
pubmed-meshheading:3267232-Cloning, Molecular,
pubmed-meshheading:3267232-Genes,
pubmed-meshheading:3267232-Genes, Regulator,
pubmed-meshheading:3267232-HeLa Cells,
pubmed-meshheading:3267232-Histones,
pubmed-meshheading:3267232-Humans,
pubmed-meshheading:3267232-Molecular Sequence Data,
pubmed-meshheading:3267232-Mutation,
pubmed-meshheading:3267232-Promoter Regions, Genetic,
pubmed-meshheading:3267232-Transcription, Genetic,
pubmed-meshheading:3267232-Transfection
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pubmed:year |
1988
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pubmed:articleTitle |
Conservation of histone H2A/H2B intergene regions: a role for the H2B specific element in divergent transcription.
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pubmed:affiliation |
Department of Biochemistry, University of Adelaide, South Australia.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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