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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1989-2-23
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pubmed:abstractText |
The synthesis and intracellular sorting of the interleukin-2 (IL-2) receptor were studied with a line of mutant Chinese hamster ovary (CHO) cells with a reversible defect in protein O glycosylation. Under normal culture conditions the mutant ldlD cannot add N-acetylgalactosamine (Ga1NAc) to proteins. Ga1NAc is the first sugar of mucin-type O-linked oligosaccharides attached to protein. This O-glycosylation defect is rapidly corrected when Ga1NAc is added to the culture mediu. An expression vector for the p55 human IL-2 receptor was transfected into wild-type CHO and ldlD cells and the structure, stability, and cell surface expression of the receptor were examined by immunoprecipitation and antibody-binding assays. Essentially all of the mature form of the normally glycosylated IL-2 receptor in both wild-type CHO cells and ldlD cells incubated with Ga1NAc was expressed on the cell surface. The stability of O-linked carbohydrate-deficient (Od) IL-2 receptors (in ldlD cells without Ga1NAc) was normal; however, missorting of the Od receptors resulted in very little cell surface expression. The sialidase sensitivity and endoglycosidase H resistance of mature Od IL-2 receptors suggest that Od receptor missorting occurred in or beyond the trans Golgi apparatus. The abnormal sorting of the Od IL-2 receptor is compared with the O-glycosylation dependence of the surface expression and stability of the low-density lipoprotein receptor, decay-accelerating factor, and the major antigen envelope protein of Epstein-Barr virus.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-2881559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-2944601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-2981876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-2989411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-3005267,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-3008337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-3010466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-3031660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-3081898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-3082877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-3086875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-3095922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-3096988,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-3097520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-3097640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-3098894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-3108887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-3155653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-3380796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-3476951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-3517003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-3700466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-3930953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-3934560,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-3948246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-4705382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-6090574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-6096720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-6237101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-6305509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-6317691,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-6321999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-6327078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-6417659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-6427923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-6436815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-6975347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-6982926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-7000676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264879-7023366
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3357-63
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3264879-Animals,
pubmed-meshheading:3264879-Cell Line,
pubmed-meshheading:3264879-Flow Cytometry,
pubmed-meshheading:3264879-Glycoproteins,
pubmed-meshheading:3264879-Glycosylation,
pubmed-meshheading:3264879-Interleukin-2,
pubmed-meshheading:3264879-Mutation,
pubmed-meshheading:3264879-Receptors, Antigen, T-Cell,
pubmed-meshheading:3264879-Receptors, Interleukin-2
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pubmed:year |
1988
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pubmed:articleTitle |
Abnormal intracellular sorting of O-linked carbohydrate-deficient interleukin-2 receptors.
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pubmed:affiliation |
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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