Linked Life Data

3264623

Source:http://linkedlifedata.com/resource/pubmed/id/3264623

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1989-2-7
pubmed:abstractText
The effects of human recombinant interleukin-1 alpha (IL-1 alpha) on proteoglycan (PG) metabolism of rabbit intervertebral disc were investigated morphologically and biochemically using rabbit annulus fibrosus (AF) cells in culture. AF cells could maintain their differentiated phenotype well in our primary culturing condition. In this situation, IL-1 alpha stimulated the cells and induced marked increase of PG release. Dose dependency of IL-1 alpha on PG release was seen in the concentration range between 5-50 U/ml. Caseinolytic activity produced and secreted into the medium by AF cells was assayed and it was found that IL-1 alpha enhanced the enzyme activities in the medium. The effects of IL-1 alpha on PG and DNA synthesis were also studied. Slight depression was observed in PG synthesis but there was no effect on DNA synthesis. These data suggest that IL-1 alpha may play an important role in PG metabolism of intervertebral disc cells, especially in the catabolic pathway of PG.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0362-2436
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1284-90
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
The role of interleukin-1 on proteoglycan metabolism of rabbit annulus fibrosus cells cultured in vitro.
pubmed:affiliation
Department of Orthopaedic Surgery, National Defense Medical College, Saitama-ken, Japan.
pubmed:publicationType
Journal Article, In Vitro