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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1988-12-28
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pubmed:abstractText |
The interaction of dansylpenicillin with the class A Staphylococcus aureus PCI beta-lactamase yielded an accumulating intermediate with fluorescence enhanced beyond that of the substrate. Acid quenching of the reaction mixture yielded a denatured enzyme with 1 molar equivalent of dansyl group covalently bound to it. A similar quenching experiment with the PC1 beta-lactamase and [14C]benzylpenicillin yielded an enzyme with 1 molar equivalent of 14C covalently bound. These data indicate that in turnover of S-type penicillins by the PC1 beta-lactamase deacylation is rate-determining. This has not indicate previously been demonstrated for a class A beta-lactamase.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264153-13882319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264153-13900849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264153-14070048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264153-220094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264153-308803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264153-3107125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264153-3115289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264153-3118942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264153-3496243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264153-3873255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264153-3933490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264153-4203115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264153-6049371,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264153-6333871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264153-638183,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264153-6605346,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264153-6773559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264153-6800840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264153-6818947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264153-6975275,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264153-6994800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264153-817286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3264153-999812
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
254
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
919-22
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1988
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pubmed:articleTitle |
Accumulation of acyl-enzyme intermediates during turnover of penicillins by the class A beta-lactamase of Staphylococcus aureus PC1.
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pubmed:affiliation |
Department of Chemistry, Wesleyan University, Middletown, CT 06457.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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