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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1989-1-6
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pubmed:abstractText |
Two distinct murine B-cell differentiation factors, designated B151-TRF1 and B151-TRF2, were described originally as B151K12 T-cell hybridoma-derived lymphokines that induce immunoglobulin (Ig) secretion by antigen-activated B cells and unstimulated B cells, respectively. In the present study, we found that a highly purified B151-TRF1 fraction prepared by reversed-phase high-performance liquid chromatography (RP-HPLC) also has the ability to cause a polyclonal differentiation of unstimulated B cells into IgM-secreting cells in the apparent absence of co-stimulant. The activity of the B151-TRF1 fraction but not the B151-TRF2 fraction on unstimulated B cells was markedly inhibited by addition of a monoclonal antibody (mAb) specific for the B151-TRF1/IL-5 to the culture. To determine whether B151-TRF1/IL-5 and B151-TRF2 act on distinct populations among unstimulated B cells, the responsiveness of neonatal B cells and adult B cells that had been fractionated by Percoll density gradient centrifugation was assessed. B151-TRF1/IL-5 predominantly acted on lower density B cells, which appeared around 3 weeks after birth in the spleen. In contrast, B151-TRF2 could activate both lower and higher density B cells almost equally and B151-TRF2-responsive B cells were already present by 1 week of age. Thus, these results suggest that B151-TRF1/IL-5 and B151-TRF2 act on distinct subpopulations among antigen-unprimed normal B cells to induce IgM-secreting cells.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3263941-15036,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3263941-2431042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3263941-2440025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3263941-2939856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3263941-2978227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3263941-3005865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3263941-302205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3263941-3125248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3263941-3487787,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3263941-3489778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3263941-3495803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3263941-3519774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3263941-3871109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3263941-3871950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3263941-3873068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3263941-3873071,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3263941-3897376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3263941-3928981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3263941-4610054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3263941-6238333,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3263941-6403867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3263941-6429034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3263941-6435125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3263941-6601148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3263941-6606666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3263941-6802927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3263941-6965395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3263941-6968266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3263941-6975305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3263941-7038025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3263941-90107
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
65
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
221-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3263941-Animals,
pubmed-meshheading:3263941-Antibodies, Monoclonal,
pubmed-meshheading:3263941-Antigens, Differentiation, B-Lymphocyte,
pubmed-meshheading:3263941-B-Lymphocytes,
pubmed-meshheading:3263941-Immunoglobulin M,
pubmed-meshheading:3263941-Interleukin-4,
pubmed-meshheading:3263941-Interleukin-5,
pubmed-meshheading:3263941-Interleukins,
pubmed-meshheading:3263941-Lymphocyte Activation,
pubmed-meshheading:3263941-Mice
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pubmed:year |
1988
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pubmed:articleTitle |
T-cell-derived factor B151-TRF1/IL-5 activates blastoid cells among unprimed B cells to induce a polyclonal differentiation into immunoglobulin M-secreting cells.
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pubmed:affiliation |
Department of Oncogenesis, Osaka University Medical School, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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