Linked Life Data

3262715

Source:http://linkedlifedata.com/resource/pubmed/id/3262715

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1988-11-8
pubmed:abstractText
Monoclonal antibodies (MAbs) were complexed to solid matrices and used to purify virus proteins from simian virus 5 (SV5)-infected tissue culture cells, ideally in such a way as to bind equimolar amounts of antigen to antibody. The resulting solid matrix-antibody-antigen (SMAA) complexes were then used as immunogens and successfully induced specific humoral and cytotoxic T cell responses. By attaching more than one MAb to the solid matrix and using such complexes to purify the respective proteins multivalent immunization was achieved. Analysis of the cytotoxic T cell response of immunized animals indicated that both surface and internal SV5 structural proteins can act as target antigens. Immunization with SMAA complexes, in the absence of adjuvant, induced higher levels of antibody than the antigen alone precipitated on alum. However, immunization with SMAA complexes resulted in relatively less antibody being produced to the antigenic determinant through which the protein is coupled, via antibody, to the SMAA complex compared with the amount of antibody produced against other antigenic determinants on that protein. The particulate solid matrix used to form the SMAA complexes in most of the experiments was a 'fixed' and killed suspension of the Cowan A strain of Staphylococcus aureus, although preliminary results indicated that Protein A-Sepharose could also be used as a solid matrix. Prior immunization with S. aureus alone did not reduce the level of the immune response to the appropriate antigen on subsequent immunization with S. aureus-antibody-antigen complexes. In fact on immunization of mice with these complexes the level of antibody produced to the S. aureus matrix itself was less when S. aureus-antibody-antigen complexes were used as immunogens than when S. aureus or S. aureus-antibody complexes were used. Furthermore, rabbits immunized with S. aureus-mouse MAb-antigen complexes showed a vigorous immune response to the antigen.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-1317
pubmed:author
pubmed:issnType
Print
pubmed:volume
69 ( Pt 10)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2505-16
pubmed:dateRevised
2009-9-29
pubmed:meshHeading
pubmed-meshheading:3262715-Animals, pubmed-meshheading:3262715-Antibodies, Monoclonal, pubmed-meshheading:3262715-Antibodies, Viral, pubmed-meshheading:3262715-Antigen-Antibody Complex, pubmed-meshheading:3262715-Cell Line, pubmed-meshheading:3262715-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:3262715-Hemagglutinins, Viral, pubmed-meshheading:3262715-Humans, pubmed-meshheading:3262715-Immunization, pubmed-meshheading:3262715-Lymphocyte Activation, pubmed-meshheading:3262715-Neuraminidase, pubmed-meshheading:3262715-Paramyxoviridae, pubmed-meshheading:3262715-Rabbits, pubmed-meshheading:3262715-Radioimmunoassay, pubmed-meshheading:3262715-Staphylococcus aureus, pubmed-meshheading:3262715-T-Lymphocytes, Cytotoxic, pubmed-meshheading:3262715-Vaccines, pubmed-meshheading:3262715-Vaccines, Synthetic, pubmed-meshheading:3262715-Viral Core Proteins, pubmed-meshheading:3262715-Viral Matrix Proteins, pubmed-meshheading:3262715-Viral Proteins, pubmed-meshheading:3262715-Viral Structural Proteins
pubmed:year
1988
pubmed:articleTitle
Humoral and cytotoxic T cell immune responses to internal and external structural proteins of simian virus 5 induced by immunization with solid matrix-antibody-antigen complexes.
pubmed:affiliation
Department of Biochemistry and Microbiology, University of St Andrews, Fife, U.K.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't